The determination of insulin in plasma and urine
We have tried to determine insulin in urine and plasma by means of aminoguanidine sulfate solution. The urine and plasma are cleared with Somogyi reagent. The filtrate is then diluted with water to the range of the aminoguanidine sulfate reagent. Our only difficulty lies in the compliance of the curves to Beer's law. The curves tend to give less color in the higher concentrations and more color in the lower concentrations thus the curves do not pass through or near the origin. It is our fond hope that if the Beer's law plots were straightened out the determination would be successful. By running determinations simultaneously at two different temperatures, it would then be possible to determine insulin and only insulin.
Thesis (M.A.)--Boston University