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Smoking-Induced Gene Expression Changes in the Bronchial Airway Are Reflected in Nasal and Buccal Epithelium

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dc.contributor.author Sridhar, Sriram en_US
dc.contributor.author Schembri, Frank en_US
dc.contributor.author Zeskind, Julie en_US
dc.contributor.author Shah, Vishal en_US
dc.contributor.author Gustafson, Adam M en_US
dc.contributor.author Steiling, Katrina en_US
dc.contributor.author Liu, Gang en_US
dc.contributor.author Dumas, Yves-Martine en_US
dc.contributor.author Zhang, Xiaohui en_US
dc.contributor.author Brody, Jerome S en_US
dc.contributor.author Lenburg, Marc E en_US
dc.contributor.author Spira, Avrum en_US
dc.date.accessioned 2011-12-29T23:59:38Z
dc.date.available 2011-12-29T23:59:38Z
dc.date.copyright 2008 en_US
dc.date.issued 2008-5-30 en_US
dc.identifier.citation Sridhar, Sriram, Frank Schembri, Julie Zeskind, Vishal Shah, Adam M Gustafson, Katrina Steiling, Gang Liu, Yves-Martine Dumas, Xiaohui Zhang, Jerome S Brody, Marc E Lenburg, Avrum Spira. "Smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium." BMC Genomics 9:259. (2008) en_US
dc.identifier.issn 1471-2164 en_US
dc.identifier.uri http://hdl.handle.net/2144/2671
dc.description.abstract BACKGROUND: Cigarette smoking is a leading cause of preventable death and a significant cause of lung cancer and chronic obstructive pulmonary disease. Prior studies have demonstrated that smoking creates a field of molecular injury throughout the airway epithelium exposed to cigarette smoke. We have previously characterized gene expression in the bronchial epithelium of never smokers and identified the gene expression changes that occur in the mainstem bronchus in response to smoking. In this study, we explored relationships in whole-genome gene expression between extrathorcic (buccal and nasal) and intrathoracic (bronchial) epithelium in healthy current and never smokers. RESULTS: Using genes that have been previously defined as being expressed in the bronchial airway of never smokers (the "normal airway transcriptome"), we found that bronchial and nasal epithelium from non-smokers were most similar in gene expression whencompared to other epithelial and nonepithelial tissues, with several antioxidant, detoxification, and structural genes being highly expressed in both the bronchus and nose. Principle component analysis of previously defined smoking-induced genes from the bronchus suggested that smoking had a similar effect on gene expression in nasal epithelium. Gene set enrichment analysis demonstrated that this set of genes was also highly enriched among the genes most altered by smoking in both nasal and buccal epithelial samples. The expression of several detoxification genes was commonly altered by smoking in all three respiratory epithelial tissues, suggesting a common airway-wide response to tobacco exposure. CONCLUSION: Our findings support a relationship between gene expression in extra- and intrathoracic airway epithelial cells and extend the concept of a smoking-induced field of injury to epithelial cells that line the mouth and nose. This relationship could potentially be utilized to develop a non-invasive biomarker for tobacco exposure as well as a non-invasive screening or diagnostic tool providing information about individual susceptibility to smoking-induced lung diseases. en_US
dc.description.sponsorship Doris Duke Charitable Foundation; National Institute of Health and National Cancer Institute (R01CA124640); National Institute of Health and National Institute of Environmental Health Sciences (U01ES016035) en_US
dc.language.iso en en_US
dc.publisher BioMed Central en_US
dc.rights Copyright 2008 Sridhar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution 2.0 License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en_US
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_US
dc.title Smoking-Induced Gene Expression Changes in the Bronchial Airway Are Reflected in Nasal and Buccal Epithelium en_US
dc.type article en_US
dc.identifier.doi 10.1186/1471-2164-9-259 en_US
dc.identifier.pubmedid 18513428 en_US
dc.identifier.pmcid 2435556 en_US


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Copyright 2008 Sridhar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution 2.0 License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as Copyright 2008 Sridhar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution 2.0 License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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