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Tissue-Specific mRNA Expression Profiling in Grape Berry Tissues

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dc.contributor.author Grimplet, Jerome en_US
dc.contributor.author Deluc, Laurent G en_US
dc.contributor.author Tillett, Richard L en_US
dc.contributor.author Wheatley, Matthew D en_US
dc.contributor.author Schlauch, Karen A en_US
dc.contributor.author Cramer, Grant R en_US
dc.contributor.author Cushman, John C en_US
dc.date.accessioned 2011-12-30T00:07:00Z
dc.date.available 2011-12-30T00:07:00Z
dc.date.copyright 2007 en_US
dc.date.issued 2007-6-21 en_US
dc.identifier.citation Grimplet, Jerome, Laurent G Deluc, Richard L Tillett, Matthew D Wheatley, Karen A Schlauch, Grant R Cramer, John C Cushman. "Tissue-specific mRNA expression profiling in grape berry tissues." BMC Genomics 8:187. (2007) en_US
dc.identifier.issn 1471-2164 en_US
dc.identifier.uri http://hdl.handle.net/2144/2680
dc.description.abstract BACKGROUND: Berries of grape (Vitis vinifera) contain three major tissue types (skin, pulp and seed) all of which contribute to the aroma, color, and flavor characters of wine. The pericarp, which is composed of the exocarp (skin) and mesocarp (pulp), not only functions to protect and feed the developing seed, but also to assist in the dispersal of the mature seed by avian and mammalian vectors. The skin provides volatile and nonvolatile aroma and color compounds, the pulp contributes organic acids and sugars, and the seeds provide condensed tannins, all of which are important to the formation of organoleptic characteristics of wine. In order to understand the transcriptional network responsible for controlling tissue-specific mRNA expression patterns, mRNA expression profiling was conducted on each tissue of mature berries of V. vinifera Cabernet Sauvignon using the Affymetrix GeneChip® Vitis oligonucleotide microarray ver. 1.0. In order to monitor the influence of water-deficit stress on tissue-specific expression patterns, mRNA expression profiles were also compared from mature berries harvested from vines subjected to well-watered or water-deficit conditions. RESULTS: Overall, berry tissues were found to express approximately 76% of genes represented on the Vitis microarray. Approximately 60% of these genes exhibited significant differential expression in one or more of the three major tissue types with more than 28% of genes showing pronounced (2-fold or greater) differences in mRNA expression. The largest difference in tissue-specific expression was observed between the seed and pulp/skin. Exocarp tissue, which is involved in pathogen defense and pigment production, showed higher mRNA abundance relative to other berry tissues for genes involved with flavonoid biosynthesis, pathogen resistance, and cell wall modification. Mesocarp tissue, which is considered a nutritive tissue, exhibited a higher mRNA abundance of genes involved in cell wall function and transport processes. Seeds, which supply essential resources for embryo development, showed higher mRNA abundance of genes encoding phenylpropanoid biosynthetic enzymes, seed storage proteins, and late embryogenesis abundant proteins. Water-deficit stress affected the mRNA abundance of 13% of the genes with differential expression patterns occurring mainly in the pulp and skin. In pulp and seed tissues transcript abundance in most functional categories declined in water-deficit stressed vines relative to well-watered vines with transcripts for storage proteins and novel (no-hit) functional assignments being over represented. In the skin of berries from water-deficit stressed vines, however, transcripts from several functional categories including general phenypropanoid and ethylene metabolism, pathogenesis-related responses, energy, and interaction with the environment were significantly over-represented. CONCLUSION: These results revealed novel insights into the tissue-specific expression mRNA expression patterns of an extensive repertoire of genes expressed in berry tissues. This work also establishes an extensive catalogue of gene expression patterns for future investigations aimed at the dissection of the transcriptional regulatory hierarchies that govern tissue-specific expression patterns associated with tissue differentiation within berries. These results also confirmed that water-deficit stress has a profound effect on mRNA expression patterns particularly associated with the biosynthesis of aroma and color metabolites within skin and pulp tissues that ultimately impact wine quality. en_US
dc.description.sponsorship National Science Foundation Plant Genome Program (DBI-0217653); University of Arizona Agricultural Experiment Station; National Institutes of Health (P20 RR-016464); National Center for Research Resources (INBRE-BRIN Program); National Insitute of Health IDeA Network of Biomedical Research Excellence (INBRE RR-03-008) en_US
dc.language.iso en en_US
dc.publisher BioMed Central en_US
dc.rights Copyright 2007 Grimplet et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution 2.0 License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en_US
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_US
dc.title Tissue-Specific mRNA Expression Profiling in Grape Berry Tissues en_US
dc.type article en_US
dc.identifier.doi 10.1186/1471-2164-8-187 en_US
dc.identifier.pubmedid 17584945 en_US
dc.identifier.pmcid 1925093 en_US


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Copyright 2007 Grimplet et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution 2.0 License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as Copyright 2007 Grimplet et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution 2.0 License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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