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The Cell Wall Teichuronic Acid Synthetase (TUAS) Is an Enzyme Complex Located in the Cytoplasmic Membrane of Micrococcus luteus

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dc.contributor.author Deng, Lingyi Lynn en_US
dc.contributor.author Alexander, Alice A. en_US
dc.contributor.author Lei, Sijin en_US
dc.contributor.author Anderson, John S. en_US
dc.date.accessioned 2012-01-09T20:54:52Z
dc.date.available 2012-01-09T20:54:52Z
dc.date.copyright 2010 en_US
dc.date.issued 2010-4-6 en_US
dc.identifier.citation Deng, Lingyi Lynn, Alice A. Alexander, Sijin Lei, John S. Anderson. "The Cell Wall Teichuronic Acid Synthetase (TUAS) Is an Enzyme Complex Located in the Cytoplasmic Membrane of Micrococcus luteus" Biochemistry Research International 2010:395758. (2010) en_US
dc.identifier.issn 2090-2255 en_US
dc.identifier.uri http://hdl.handle.net/2144/2913
dc.description.abstract The cell wall teichuronic acid (TUA) of Micrococcus luteus is a long-chain polysaccharide composed of disaccharide repeating units [-4-β-D-ManNAcAp-(1→6)α-D-Glcp−1-]n, which is covalently anchored to the peptidoglycan on the inner cell wall and extended to the outer surface of the cell envelope. An enzyme complex responsible for the TUA chain biosynthesis was purified and characterized. The 440kDa enzyme complex, named teichuronic acid synthetase (TUAS), is an octomer composed of two kinds of glycosyltransferases, Glucosyltransferase, and ManNAcA-transferase, which is capable of catalyzing the transfer of disaccharide glycosyl residues containing both glucose and the N-acetylmannosaminuronic acid residues. TUAS displays hydrophobic properties and is found primarily associated with the cytoplasmic membrane. The purified TUAS contains carotinoids and lipids. TUAS activity is diminished by phospholipase digestion. We propose that TUAS serves as a multitasking polysaccharide assembling station on the bacterial membrane. en_US
dc.description.sponsorship National Institute of Allergy and Infectious Diseases (Public Health Service Grants AI-08295); American Lung Association (RG-107-N) en_US
dc.language.iso en en_US
dc.publisher Hindawi Publishing Corporation en_US
dc.rights Copyright 2010 Lingyi Lynn Deng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en_US
dc.title The Cell Wall Teichuronic Acid Synthetase (TUAS) Is an Enzyme Complex Located in the Cytoplasmic Membrane of Micrococcus luteus en_US
dc.type article en_US
dc.identifier.doi 10.1155/2010/395758 en_US
dc.identifier.pubmedid 21188072 en_US
dc.identifier.pmcid 3005890 en_US


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