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Reduced Influenza Viral Neutralizing Activity of Natural Human Trimers of Surfactant Protein D

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dc.contributor.author Hartshorn, Kevan L en_US
dc.contributor.author White, Mitchell R en_US
dc.contributor.author Tecle, Tesfaldet en_US
dc.contributor.author Tornoe, Ida en_US
dc.contributor.author Sorensen, Grith L en_US
dc.contributor.author Crouch, Erika C en_US
dc.contributor.author Holmskov, Uffe en_US
dc.date.accessioned 2012-01-09T21:04:09Z
dc.date.available 2012-01-09T21:04:09Z
dc.date.copyright 2007 en_US
dc.date.issued 2007-2-5 en_US
dc.identifier.citation Hartshorn, Kevan L, Mitchell R White, Tesfaldet Tecle, Ida Tornoe, Grith L Sorensen, Erika C Crouch, Uffe Holmskov. "Reduced influenza viral neutralizing activity of natural human trimers of surfactant protein D" Respiratory Research 8(1):9. (2007) en_US
dc.identifier.issn 1465-993X en_US
dc.identifier.uri http://hdl.handle.net/2144/2989
dc.description.abstract BACKGROUND. Surfactant protein D (SP-D) plays important roles in innate host defense against influenza A virus (IAV) infection. Common human polymorphisms of SP-D have been found in many human populations and associated with increased risk of certain infections. We recently reported that the Thr/Thr 11 form of SP-D is associated with low serum levels and assembles predominantly as trimers as opposed to the more common multimeric forms of SP-D. METHODS. Preliminary experiments were done to establish the effects of different monoclonal antibodies against SP-D on ability of SP-D to bind to or neutralize the virus. We then purified natural human trimeric and multimeric forms of SP-D from amniotic fluid and tested ability of these preparations to bind to IAV, to inhibit infectivity and hemagglutination activity of IAV in vitro. RESULTS. In initial experiments mAbs directed against different areas on the CRD of SP-D were found to have differing effects on antiviral activity. Using an mAb that did not interfere with antiviral activity of SP-D, we confirm that natural SP-D trimers had reduced ability to bind to IAV. In addition, the trimers had reduced ability to neutralize IAV as compared to natural human SP-D multimers as well as reduced hemagglutination inhibiting activity against several strains of IAV. Natural SP-D trimers also had different interactions with human neutrophil peptide defensins (HNPs) in viral neutralization assays as compared to multimeric SP-D. CONCLUSION. These studies indicate that a common human polymorphic form of SP-D may modulate host defense against IAV and give impetus to clinical studies correlating this genotype with risk for IAV infection in susceptible groups. We also show that mAbs directed against different areas on the carbohydrate recognition domain of SP-D can be useful for dissecting out different functional properties of the protein. en_US
dc.language.iso en en_US
dc.publisher BioMed Central en_US
dc.rights Copyright 2007 Hartshorn et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en_US
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_US
dc.title Reduced Influenza Viral Neutralizing Activity of Natural Human Trimers of Surfactant Protein D en_US
dc.type article en_US
dc.identifier.doi 10.1186/1465-9921-8-9 en_US
dc.identifier.pubmedid 17280604 en_US
dc.identifier.pmcid 1797806 en_US


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Copyright 2007 Hartshorn et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as Copyright 2007 Hartshorn et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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