Secondary structure effects on DNA hybridization kinetics: a solution versus surface comparison

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dc.contributor.author Gao, Yang en_US
dc.contributor.author Wolf, Lauren K. en_US
dc.contributor.author Georgiadis, Rosina M. en_US
dc.date.accessioned 2009-04-13T23:23:26Z
dc.date.available 2009-04-13T23:23:26Z
dc.date.issued 2006 en_US
dc.identifier.citation 2006. "Secondary structure effects on DNA hybridization kinetics: a solution versus surface comparison," Nucleic Acids Research. vol. 34 issue. 11 . en_US
dc.identifier.other PMC1488884 en_US
dc.identifier.uri 10.1093/nar/gkl422 en_US
dc.identifier.uri http://hdl.handle.net/2144/1006
dc.description.abstract The hybridization kinetics for a series of designed 25mer probe�target pairs having varying degrees of secondary structure have been measured by UV absorbance and surface plasmon resonance (SPR) spectroscopy in solution and on the surface, respectively. Kinetic rate constants derived from the resultant data decrease with increasing probe and target secondary structure similarly in both solution and surface environments. Specifically, addition of three intramolecular base pairs in the probe and target structure slow hybridization by a factor of two. For individual strands containing four or more intramolecular base pairs, hybridization cannot be described by a traditional two-state model in solution-phase nor on the surface. Surface hybridization rates are also 20- to 40-fold slower than solution-phase rates for identical sequences and conditions. These quantitative findings may have implications for the design of better biosensors, particularly those using probes with deliberate secondary structure. en_US
dc.relation.ispartof Nucleic Acids Research en_US
dc.relation.ispartofseries vol. 34 issue. 11 en_US
dc.title Secondary structure effects on DNA hybridization kinetics: a solution versus surface comparison en_US
dc.type article en_US

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