Reversible and Permanent Effects of Tobacco Smoke Exposure on Airway Epithelial Gene Expression

OpenBU

Show simple item record

dc.contributor.author Beane, Jennifer en_US
dc.contributor.author Sebastiani, Paola en_US
dc.contributor.author Liu, Gang en_US
dc.contributor.author Brody, Jerome S en_US
dc.contributor.author Lenburg, Marc E en_US
dc.contributor.author Spira, Avrum en_US
dc.date.accessioned 2012-01-09T14:44:45Z
dc.date.available 2012-01-09T14:44:45Z
dc.date.copyright 2007 en_US
dc.date.issued 2007-09-25 en_US
dc.identifier.citation Beane, Jennifer, Paola Sebastiani, Gang Liu, Jerome S Brody, Marc E Lenburg, Avrum Spira. "Reversible and Permanent Effects of Tobacco Smoke Exposure on Airway Epithelial Gene Expression" Genome Biology 8(9):R201. (2007) en_US
dc.identifier.issn 1465-6914 en_US
dc.identifier.uri http://hdl.handle.net/2144/2794
dc.description.abstract Oligonucleotide microarray analysis revealed 175 genes that are differentially expressed in large airway epithelial cells of people who currently smoke compared with those who never smoked, with 28 classified as irreversible, 6 as slowly reversible, and 139 as rapidly reversible. BACKGROUND. Tobacco use remains the leading preventable cause of death in the US. The risk of dying from smoking-related diseases remains elevated for former smokers years after quitting. The identification of irreversible effects of tobacco smoke on airway gene expression may provide insights into the causes of this elevated risk. RESULTS. Using oligonucleotide microarrays, we measured gene expression in large airway epithelial cells obtained via bronchoscopy from never, current, and former smokers (n = 104). Linear models identified 175 genes differentially expressed between current and never smokers, and classified these as irreversible (n = 28), slowly reversible (n = 6), or rapidly reversible (n = 139) based on their expression in former smokers. A greater percentage of irreversible and slowly reversible genes were down-regulated by smoking, suggesting possible mechanisms for persistent changes, such as allelic loss at 16q13. Similarities with airway epithelium gene expression changes caused by other environmental exposures suggest that common mechanisms are involved in the response to tobacco smoke. Finally, using irreversible genes, we built a biomarker of ever exposure to tobacco smoke capable of classifying an independent set of former and current smokers with 81% and 100% accuracy, respectively. CONCLUSION. We have categorized smoking-related changes in airway gene expression by their degree of reversibility upon smoking cessation. Our findings provide insights into the mechanisms leading to reversible and persistent effects of tobacco smoke that may explain former smokers increased risk for developing tobacco-induced lung disease and provide novel targets for chemoprophylaxis. Airway gene expression may also serve as a sensitive biomarker to identify individuals with past exposure to tobacco smoke. en_US
dc.description.sponsorship Doris Duke Charitable Foundation; National Institutes of Health, National Cancer Institute (R21CA10650, R01CA124640); National Institute of Environmental Health Sciences (U01 ES016035) en_US
dc.language.iso en en_US
dc.publisher BioMed Central en_US
dc.rights Copyright 2007 Beane et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en_US
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en_US
dc.title Reversible and Permanent Effects of Tobacco Smoke Exposure on Airway Epithelial Gene Expression en_US
dc.type article en_US
dc.identifier.doi 10.1186/gb-2007-8-9-r201 en_US
dc.identifier.pubmedid 17894889 en_US
dc.identifier.pmcid 2375039 en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search OpenBU


Advanced Search

Browse

Deposit Materials