Bronchus-Associated Lymphoid Tissue (BALT) and Survival in a Vaccine Mouse Model of Tularemia

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dc.contributor.author Chiavolini, Damiana en_US
dc.contributor.author Rangel-Moreno, Javier en_US
dc.contributor.author Berg, Gretchen en_US
dc.contributor.author Christian, Kate en_US
dc.contributor.author Oliveira-Nascimento, Laura en_US
dc.contributor.author Weir, Susan en_US
dc.contributor.author Alroy, Joseph en_US
dc.contributor.author Randall, Troy D. en_US
dc.contributor.author Wetzler, Lee M. en_US
dc.date.accessioned 2012-01-09T21:00:24Z
dc.date.available 2012-01-09T21:00:24Z
dc.date.issued 2010-6-16 en_US
dc.identifier.citation Chiavolini, Damiana, Javier Rangel-Moreno, Gretchen Berg, Kate Christian, Laura Oliveira-Nascimento, Susan Weir, Joseph Alroy, Troy D. Randall, Lee M. Wetzler. "Bronchus-Associated Lymphoid Tissue (BALT) and Survival in a Vaccine Mouse Model of Tularemia" PLoS ONE 5(6): e11156. (2010) en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://hdl.handle.net/2144/2980
dc.description.abstract BACKGROUND. Francisella tularensis causes severe pulmonary disease, and nasal vaccination could be the ideal measure to effectively prevent it. Nevertheless, the efficacy of this type of vaccine is influenced by the lack of an effective mucosal adjuvant. METHODOLOGY/PRINCIPAL FINDINGS. Mice were immunized via the nasal route with lipopolysaccharide isolated from F. tularensis and neisserial recombinant PorB as an adjuvant candidate. Then, mice were challenged via the same route with the F. tularensis attenuated live vaccine strain (LVS). Mouse survival and analysis of a number of immune parameters were conducted following intranasal challenge. Vaccination induced a systemic antibody response and 70% of mice were protected from challenge as showed by their improved survival and weight regain. Lungs from mice recovering from infection presented prominent lymphoid aggregates in peribronchial and perivascular areas, consistent with the location of bronchus-associated lymphoid tissue (BALT). BALT areas contained proliferating B and T cells, germinal centers, T cell infiltrates, dendritic cells (DCs). We also observed local production of antibody generating cells and homeostatic chemokines in BALT areas. CONCLUSIONS. These data indicate that PorB might be an optimal adjuvant candidate for improving the protective effect of F. tularensis antigens. The presence of BALT induced after intranasal challenge in vaccinated mice might play a role in regulation of local immunity and long-term protection, but more work is needed to elucidate mechanisms that lead to its formation. en_US
dc.description.sponsorship National Institute of Allergy and Infectious Diseases (U19 AI056543); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Conselho Nacional de Desenvolvimento Científico e Tecnológico en_US
dc.language.iso en en_US
dc.publisher Public Library of Science en_US
dc.rights Chiavolini et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_US
dc.title Bronchus-Associated Lymphoid Tissue (BALT) and Survival in a Vaccine Mouse Model of Tularemia en_US
dc.type article en_US
dc.identifier.doi 10.1371/journal.pone.0011156 en_US
dc.identifier.pubmedid 20585390 en_US
dc.identifier.pmcid 2886834 en_US

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