Practical Implementation of Log-Scale Active Illumination Microscopy


Show simple item record K. Chu, Kengyeh en_US Lim, Daryl en_US Mertz, Jerome en_US 2012-01-11T00:39:11Z 2012-01-11T00:39:11Z 2010 en_US 2010-7-16 en_US
dc.identifier.citation K. Chu, Kengyeh, Daryl Lim, Jerome Mertz. "Practical implementation of log-scale active illumination microscopy" Biomedical Optics Express 1(1): 236-245. (2010) en_US
dc.identifier.issn 2156-7085 en_US
dc.description.abstract Active illumination microscopy (AIM) is a method of redistributing dynamic range in a scanning microscope using real-time feedback to control illumination power on a sub-pixel time scale. We describe and demonstrate a fully integrated instrument that performs both feedback and image reconstruction. The image is reconstructed on a logarithmic scale to accommodate the dynamic range benefits of AIM in a single output channel. A theoretical and computational analysis of the influence of noise on active illumination feedback is presented, along with imaging examples illustrating the benefits of AIM. While AIM is applicable to any type of scanning microscope, we apply it here specifically to two-photon microscopy. en_US
dc.description.sponsorship National Institutes of Health en_US
dc.language.iso en en_US
dc.publisher Optical Society of America en_US
dc.rights Copyright 2010 Optical Society of America This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. en_US
dc.rights.uri en_US
dc.subject (110.0180) Microscopy en_US
dc.subject (180.2520) Fluorescence microscopy en_US
dc.subject (180.5810) Scanning microscopy en_US
dc.subject (190.4180) Multiphoton processes en_US
dc.title Practical Implementation of Log-Scale Active Illumination Microscopy en_US
dc.type article en_US
dc.identifier.doi 10.1364/BOE.1.000236 en_US
dc.identifier.pmcid 3005158 en_US

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