Effector Caspase Dcp-1 and IAP Protein Bruce Regulate Starvation-Induced Autophagy during Drosophila Melanogaster Oogenesis

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dc.contributor.author Hou, Ying-Chen Claire en_US
dc.contributor.author Chittaranjan, Suganthi en_US
dc.contributor.author Barbosa, Sharon González en_US
dc.contributor.author McCall, Kimberly en_US
dc.contributor.author Gorski, Sharon M. en_US
dc.date.accessioned 2012-01-11T16:42:56Z
dc.date.available 2012-01-11T16:42:56Z
dc.date.issued 2008-09-15 en_US
dc.identifier.citation Hou, Ying-Chen Claire, Suganthi Chittaranjan, Sharon González Barbosa, Kimberly McCall, Sharon M. Gorski. "Effector caspase Dcp-1 and IAP protein Bruce regulate starvation-induced autophagy during Drosophila melanogaster oogenesis" The Journal of Cell Biology 182(6): 1127-1139. (2008) en_US
dc.identifier.issn 1540-8140 en_US
dc.identifier.uri http://hdl.handle.net/2144/3120
dc.description.abstract A complex relationship exists between autophagy and apoptosis, but the regulatory mechanisms underlying their interactions are largely unknown. We conducted a systematic study of Drosophila melanogaster cell death–related genes to determine their requirement in the regulation of starvation-induced autophagy. We discovered that six cell death genes—death caspase-1 (Dcp-1), hid, Bruce, Buffy, debcl, and p53—as well as Ras–Raf–mitogen activated protein kinase signaling pathway components had a role in autophagy regulation in D. melanogaster cultured cells. During D. melanogaster oogenesis, we found that autophagy is induced at two nutrient status checkpoints: germarium and mid-oogenesis. At these two stages, the effector caspase Dcp-1 and the inhibitor of apoptosis protein Bruce function to regulate both autophagy and starvation-induced cell death. Mutations in Atg1 and Atg7 resulted in reduced DNA fragmentation in degenerating midstage egg chambers but did not appear to affect nuclear condensation, which indicates that autophagy contributes in part to cell death in the ovary. Our study provides new insights into the molecular mechanisms that coordinately regulate autophagic and apoptotic events in vivo. en_US
dc.description.sponsorship Canadian Institutes of Health (MOP-78882); National Institutes of Health (R01 GM60574); Summer Undergraduate Research Fellowship program at Boston University; National Science Foundation (0450339) en_US
dc.language.iso en en_US
dc.publisher The Rockefeller University Press en_US
dc.rights Copyright 2008 Hou et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). en_US
dc.title Effector Caspase Dcp-1 and IAP Protein Bruce Regulate Starvation-Induced Autophagy during Drosophila Melanogaster Oogenesis en_US
dc.type article en_US
dc.identifier.doi 10.1083/jcb.200712091 en_US
dc.identifier.pubmedid 18794330 en_US
dc.identifier.pmcid 2542474 en_US

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