Increased Neuronal Nitric Oxide Synthase Activity in Retinal Neurons in Early Diabetic Retinopathy

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dc.contributor.author Giove, Thomas J. en_US
dc.contributor.author Deshpande, Monika M. en_US
dc.contributor.author Gagen, Christine S. en_US
dc.contributor.author Eldred, William D. en_US
dc.date.accessioned 2012-01-11T16:52:18Z
dc.date.available 2012-01-11T16:52:18Z
dc.date.copyright 2009 en_US
dc.date.issued 2009-11-09 en_US
dc.identifier.citation Giove, Thomas J., Monika M. Deshpande, Christine S. Gagen, William D. Eldred. "Increased neuronal nitric oxide synthase activity in retinal neurons in early diabetic retinopathy" Molecular Vision 15:2249-2258. (2009) en_US
dc.identifier.issn 1090-0535 en_US
dc.identifier.uri http://hdl.handle.net/2144/3125
dc.description.abstract PURPOSE. There are increased levels of nitric oxide (NO) in diabetic retinas. The purpose of this study was to determine the extent that neuronal nitric oxide synthase (nNOS) contributes to the increased levels of retinal NO in early diabetic retinopathy by examining the expression and activity of nNOS in retinal neurons after 5 weeks of diabetes. METHODS. Changes in NO levels were measured using NO imaging of retinal neurons in mice with streptozotocin-induced diabetes for five weeks. NO imaging was compared to nNOS localization using immunocytochemistry, and nNOS message and protein levels were measured using quantitative real-time PCR and western blots. RESULTS. There was a close anatomic correlation between the localization of the increased NO production and the nNOS immunoreactivity in the retinal plexiform layers of diabetic retinas. There was no change in nNOS message, but nNOS protein was decreased and its subcellular localization was altered. Treatment with insulin or aminoguanidine partially ameliorated the increase in NO in diabetic retinas. CONCLUSIONS. These results suggest that increased nNOS activity is responsible for the majority of increased NO in retinal neurons in early diabetic retinopathy. This supports a role for increased nNOS activity in the early neuronal dysfunction in the diabetic retina. en_US
dc.description.sponsorship National Institutes of Health (NEI EY04785 to WDE) en_US
dc.language.iso en en_US
dc.publisher Molecular Vision en_US
dc.rights.uri http://creativecommons.org/licenses/by/3.0/ en_US
dc.title Increased Neuronal Nitric Oxide Synthase Activity in Retinal Neurons in Early Diabetic Retinopathy en_US
dc.type article en_US
dc.identifier.pubmedid 19936028 en_US
dc.identifier.pmcid 2776346 en_US

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