Inhibition of Influenza M2-Induced Cell Death Alleviates Its Negative Contribution to Vaccination Efficiency

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dc.contributor.author Ilyinskii, Petr O. en_US
dc.contributor.author Gambaryan, Alexandra S. en_US
dc.contributor.author Meriin, Anatoli B. en_US
dc.contributor.author Gabai, Vladimir en_US
dc.contributor.author Kartashov, Alex en_US
dc.contributor.author Thoidis, Galini en_US
dc.contributor.author Shneider, Alexander M. en_US
dc.date.accessioned 2012-01-11T21:40:12Z
dc.date.available 2012-01-11T21:40:12Z
dc.date.issued 2008-1-16 en_US
dc.identifier.citation Ilyinskii, Petr O., Alexandra S. Gambaryan, Anatoli B. Meriin, Vladimir Gabai, Alex Kartashov, Galini Thoidis, Alexander M. Shneider. "Inhibition of Influenza M2-Induced Cell Death Alleviates Its Negative Contribution to Vaccination Efficiency" PLoS ONE3(1): 1417. (2008) en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://hdl.handle.net/2144/3227
dc.description.abstract The effectiveness of recombinant vaccines encoding full-length M2 protein of influenza virus or its ectodomain (M2e) have previously been tested in a number of models with varying degrees of success. Recently, we reported a strong cytotoxic effect exhibited by M2 on mammalian cells in vitro. Here we demonstrated a decrease in protection when M2 was added to a DNA vaccination regimen that included influenza NP. Furthermore, we have constructed several fusion proteins of conserved genes of influenza virus and tested their expression in vitro and protective potential in vivo. The four-partite NP-M1-M2-NS1 fusion antigen that has M2 sequence engineered in the middle part of the composite protein was shown to not be cytotoxic in vitro. A three-partite fusion protein (consisting of NP, M1 and NS1) was expressed much more efficiently than the four-partite protein. Both of these constructs provided statistically significant protection upon DNA vaccination, with construct NP-M1-M2-NS1 being the most effective. We conclude that incorporation of M2 into a vaccination regimen may be beneficial only when its apparent cytotoxicity-linked negative effects are neutralized. The possible significance of this data for influenza vaccination regimens and preparations is discussed. en_US
dc.description.sponsorship Cure Lab, Inc. en_US
dc.language.iso en en_US
dc.publisher Public Library of Science en_US
dc.title Inhibition of Influenza M2-Induced Cell Death Alleviates Its Negative Contribution to Vaccination Efficiency en_US
dc.type article en_US
dc.identifier.doi 10.1371/journal.pone.0001417 en_US
dc.identifier.pubmedid 18197240 en_US
dc.identifier.pmcid 2175529 en_US

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