Cooperative Stimulation of Dendritic Cells by Cryptococcus neoformans Mannoproteins and CpG Oligodeoxynucleotides

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dc.contributor.author Dan, Jennifer M. en_US
dc.contributor.author Wang, Jennifer P. en_US
dc.contributor.author Lee, Chrono K. en_US
dc.contributor.author Levitz, Stuart M. en_US
dc.date.accessioned 2012-01-12T17:07:24Z
dc.date.available 2012-01-12T17:07:24Z
dc.date.issued 2008-4-30 en_US
dc.identifier.citation Dan, Jennifer M., Jennifer P. Wang, Chrono K. Lee, Stuart M. Levitz. "Cooperative Stimulation of Dendritic Cells by Cryptococcus neoformans Mannoproteins and CpG Oligodeoxynucleotides" PLoS ONE 3(4): e2046. (2008) en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://hdl.handle.net/2144/3385
dc.description.abstract While mannosylation targets antigens to mannose receptors on dendritic cells (DC), the resultant immune response is suboptimal. We hypothesized that the addition of toll-like receptor (TLR) ligands would enhance the DC response to mannosylated antigens. Cryptococcus neoformans mannoproteins (MP) synergized with CpG-containing oligodeoxynucleotides to stimulate enhanced production of proinflammatory cytokines and chemokines from murine conventional and plasmacytoid DC. Synergistic stimulation required the interaction of mannose residues on MP with the macrophage mannose receptor (MR), CD206. Moreover, synergy with MP was observed with other TLR ligands, including tripalmitoylated lipopeptide (Pam3CSK4), polyinosine-polycytidylic acid (pI:C), and imiquimod. Finally, CpG enhanced MP-specific MHC II-restricted CD4+ T-cell responses by a mechanism dependent upon DC expression of CD206 and TLR9. These data suggest a rationale for vaccination strategies that combine mannosylated antigens with TLR ligands and imply that immune responses to naturally mannosylated antigens on pathogens may be greatly augmented if TLR and MR are cooperatively stimulated. en_US
dc.description.sponsorship National Institutes of Health (RO1 AI25780, RO1 AI37532, K08 AI 53542) en_US
dc.language.iso en en_US
dc.publisher Public Library of Science en_US
dc.title Cooperative Stimulation of Dendritic Cells by Cryptococcus neoformans Mannoproteins and CpG Oligodeoxynucleotides en_US
dc.type article en_US
dc.identifier.doi 10.1371/journal.pone.0002046 en_US
dc.identifier.pubmedid 18446192 en_US
dc.identifier.pmcid 2297515 en_US

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