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dc.contributor.authorFeizpour, Amin
dc.contributor.authorReinhard, Björn
dc.date.accessioned2015-07-13T18:22:19Z
dc.date.available2015-07-13T18:22:19Z
dc.date.issued2015-03-31
dc.identifier.urihttps://hdl.handle.net/2144/11660
dc.description.abstractPhosphatidylserine (PS) and monosialotetrahexosylganglioside (G_M1) are examples of two host-derived lipids in the membrane of enveloped virus particles that are known to contribute to virus attachment, uptake, and ultimately dissemination. A quantitative characterization of their contribution to the functionality of the virus requires information about their relative concentrations in the viral membrane. Here, a gold nanoparticle (NP) binding assay for probing relative PS and G_M1 lipid concentrations in the outer leaflet of different HIV-1 and Ebola virus-like particles (VLPs) using sample sizes of less than 3×10^6 particles is introduced. The assay evaluates both scattering intensity and resonance wavelength and determines relative NP densities through plasmon coupling as a measure for the target lipid concentrations in the NP-labeled VLP membrane. In addition, the mechanical properties of the viral membrane have been found to be contributing to the efficient reproduction cycle of the virus. Membrane fluidity which is a function of temperature and membrane composition is one of the crucial factors in viral activity. We have used temporally-resolved microscopy on silver NPs to track these molecular dynamics.en_US
dc.language.isoen_USen_US
dc.titleQuantification and a Molecular Dynamics Study of Viral Membrane Lipids through Plasmon Coupling Microscopyen_US
dc.typePresentationen_US


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