Improving DNA evidence collection via quantitative analysis: a systems approach

Abstract

When collecting biological evidence from a crime scene, it is important to determine the most effective and robust collection method to ensure maximum DNA recovery. Some common biological collection methods include swabbing, cutting, scraping, and taping. Although these techniques have been a mainstay of forensic analysis, each of these methods have significant drawbacks, which include but are not limited to, the lack of surface area that may be processed, possible co-elution of PCR inhibitors, and non-optimized elution of cells from the substrate into solution. Therefore, a technique designed to optimize biological collection from items of interest, particularly large items, is necessary and not currently available for forensic use.