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dc.contributor.authorLongmire, Tyler Aldenen_US
dc.date.accessioned2015-08-05T00:52:05Z
dc.date.available2015-08-05T00:52:05Z
dc.date.issued2012
dc.date.submitted2012
dc.identifier.other(ALMA)contemp
dc.identifier.urihttps://hdl.handle.net/2144/12491
dc.descriptionThesis (Ph.D.)--Boston University PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.en_US
dc.description.abstractDuring early lung and thyroid development two populations of Nkx2-1-expressing progenitors in the foregut endoderm give rise to the entire lung and thyroid epithelium. To date, little is known about the genetic programs of these progenitors since they are few in number, present only fleetingly in the embryo, and have not been studied in pure preparations. We demonstrate here the purification, and directed differentiation in culture of primordial lung and thyroid progenitors derived from mouse embryonic stem (ES) cells. We find that sequential stage-specific and time-dependent inhibition of Transforming Growth Factor β and Bone Morphogenetic Protein signaling, followed by combinatorial stimulation of BMP and FGF signaling is necessary for the efficient specification of these cells from definitive endodermal precursors in vitro. Employing a novel Nkx2-1GFP knock-in reporter ES cell line, these progenitors can be isolated, expanded in culture, and display a global transcriptome that overlaps that of an in vivo developing lung epithelium. Moreover, these progenitors can be induced to sequentially express a broad repertoire of lineage markers indicative of the full diversity of lung and thyroid epithelial differentiation. Thus, we have derived the first population of progenitors that recapitulates the developmental milestones of lung/thyroid development.en_US
dc.language.isoen_US
dc.publisherBoston Universityen_US
dc.titleEfficient derivation of purified lung and thyroid progenitors from embryonic stem cellsen_US
dc.typeThesis/Dissertationen_US
etd.degree.nameDoctor of Philosophyen_US
etd.degree.leveldoctoralen_US
etd.degree.disciplineCell and Molecular Biologyen_US
etd.degree.grantorBoston Universityen_US


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