Hypoxia and the activity of neutrophils
Medeiros, Kyle Andrew
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Polymorphonuclear leukocytes (neutrophils) play a major role in fighting off invading bacteria. They are the first of the inflammatory cells to migrate to sites of inflammation through a process known as chemotaxis. Neutrophils eliminate invading organisms is by phagocytosis. Each phagocytic event results in the accumulation of reactive oxygen species such as the superoxide. Under hypoxic conditions associated with inflammation at local tissues, a set of transcription factors known as hypoxia-inducible factors are activated. This family of transcription factors consists primarily of two members, hypoxia inducible factor-1 (HIF-1) and hypoxia inducible factor-2 (HIF-2). HIF-1 is a heterodimer composed of alpha and beta subsets. In this study, we examined the expression of HIF-1α, HIF-1β and HIF-2 in neutrophils under hypoxic conditions at different time intervals. Expression of HIF -1α significantly increased under hypoxic conditions as compared to the normoxia. The results provide evidence suggesting that HIF-1α is the primary factor in mediating oxygen homeostasis under hypoxic conditions by regulating superoxide generation in neutrophils.
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