2013 REU Poster: Small Molecule Evolution: A Biomimetic Approach to Small Molecule Lead Generation and Optimization
Bloodworth, Tiara L.
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The goal of my project was to determine the standard calibration curves of compounds in the small molecule evolution library. Solutions of several small molecules were prepared at various concentrations and analyzed using the nanoAcquity UPLC instrument. Diode array traces were collected for each solution, in which UV-active compounds showed up as a single peak in the chromatogram. These peaks were extracted at a single wavelength (usually max) to produce new traces, and peak area was determined. Plots of peak area against concentration were obtained for each small molecule, and the results showed a linear relationship between concentration and peak area at a single wavelength. Equations of the line were found and this data will be used to determine unknown concentrations of small molecules in solution after chemical reactions are performed. The information will be vital for the biological assessment of new compounds formed by chemical modification.
Poster presentation at REU Summer's End Research Symposium, 2013, by REU participant Tiara Bloodworth, MassBay Community College - Aaron Beeler group, Dr. Kristen Mascall lab mentor.