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dc.contributor.authorVo, Hung
dc.date.accessioned2016-02-11T18:22:58Z
dc.date.issued2014
dc.identifier.urihttps://hdl.handle.net/2144/14388
dc.description.abstractB1 B cells have received increasing attention recently due to their newly discovered phagocytic and microbicidal capabilities. Several studies have demonstrated that B1 cells can phagocytize polystyrene fluorescent particles, bacteria (Staphylococcus aureus, Escherichia coli), and even apoptotic cells. Nevertheless, little is known about the biological significance of this seemingly redundant function of B1 B cells as compared to that of conventional phagocytes. Here we investigate the unique phosphotidylcholine (PtC)-specific B1 B cell phagocytosis. PtC is a major phospholipid in the biological membrane and a classical antigen recognized by B1 B cell-derived natural antibodies. These antibodies play important roles in immune defense as well as tissue homeostasis. Here we report that B1 cells preferentially phagocytose PtC-coated beads, differing from that of conventional macrophages. We further attest that these beads were truly internalized and subsequently fused with hydrolytic lysosomes indicated by increasing fluorescent intensity of a pH-sensitive dye. Despite the differences in antigen specificity, phagocytosis of both B1 cells and macrophages can be inhibited by the microtubule-inhibitor, Colchicine, in a dose-dependent manner. Most intriguingly, upon chronic high-fat diet (HFD) consumption by the host, B1 cell phagocytosis starts to lose antigen-specificity for PtC. Morphologically, some of these B1 B cells in DIO mice show enlarged cytosol and engulfed more beads, indicating a transition to macrophage-like cells. Our study suggests for the first time that B1 B cells have unique phospholipid-specific phagocytosis capacity, which is affected by diet-induced obesity.en_US
dc.language.isoen_USen_US
dc.subjectImmunologyen_US
dc.subjectB1 B cellsen_US
dc.subjectHigh fat dieten_US
dc.subjectMacrophagesen_US
dc.subjectObesityen_US
dc.subjectPhagocytosisen_US
dc.subjectPhospholipiden_US
dc.titleDysregulation of of phospholipid-specific phagocytosis by B1 B cells in diet-induced obese miceen_US
dc.typeThesis/Dissertation
dc.date.updated2016-01-22T18:56:44Z
etd.degree.nameMaster of Scienceen_US
etd.degree.levelmastersen_US
etd.degree.disciplineMedical Sciencesen_US
etd.degree.grantorBoston Universityen_US


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