L2PB1 cell depletion with diphtheria toxin in PD-L2 KIKO mice
Lee, Rebecca Arwyn
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As we learn more about immune cell subpopulations, we find an increasingly complex system of cells with diverse functions. L2pB1 cells are a PD-L2 positive B1a B lymphocyte subpopulation that has unusual properties and characteristics that are not fully understood by many. By creating and implementing a transgenic mouse model that allows for targeted depletion of this specific group of cells, we can further elucidate their physiological functions and roles in both healthy and diseased states. Here we demonstrate the depletion of L2pB1 cells utilizing a transgenic mouse model expressing Diphtheria Toxin Receptors on their surface. After a course of 4 injections of 25ng of Diphtheria Toxin per gram bodyweight, we observed a successful depletion of L2pB1 cell population. Further studies are underway investigating the effects of a high fat diet on these L2pB1 depleted mice.
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