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dc.contributor.authorJiang, Fannyen_US
dc.date.accessioned2016-04-27T18:00:59Z
dc.date.available2016-04-27T18:00:59Z
dc.date.issued2015
dc.identifier.urihttps://hdl.handle.net/2144/16107
dc.description.abstractPoly N-acetyl glucosamine is a cell surface polysaccharide that has been characterized in Staphylococcus epidermidis and Staphylococcus aureus as involved in biofilm formation and implicated in virulence. Its role in bacterial colonization and pathogenicity is now being characterized in Streptococcus pneumoniae. The aim of this thesis was to produce PNAG-deficient S. pneumoniae mutants and to confirm differential levels of biofilm formation in PNAG-deficient mutants in comparison to their wild type strains. Using PCR, gel electrophoresis, and indirect immunofluorescence, successful transformations of PNAG-deficient mutants were confirmed. Biofilm assays provided preliminary data for further investigation of the role that PNAG plays in colonization in S. pneumoniae. A novel finding in PNAG genetic structure in S. pneumoniae was also discovered, providing a new avenue of research on PNAG.en_US
dc.language.isoen_US
dc.subjectMicrobiologyen_US
dc.subjectBiofilmen_US
dc.subjectStreptococcus pneumoniaeen_US
dc.titleCharacterization of the role that bacterial surface polysaccharide poly N-acetyl glucosamine plays in nonvaccine serotypes of streptococcus pneumoniae colonization and pathogenicityen_US
dc.typeThesis/Dissertationen_US
dc.date.updated2016-04-08T20:15:50Z
etd.degree.nameMaster of Scienceen_US
etd.degree.levelmastersen_US
etd.degree.disciplineMedical Sciencesen_US
etd.degree.grantorBoston Universityen_US


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