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dc.contributor.authorYang, Chen-Yuan Charlieen_US
dc.date.accessioned2016-06-27T19:23:01Z
dc.date.available2016-06-27T19:23:01Z
dc.date.issued2016
dc.identifier.urihttps://hdl.handle.net/2144/16738
dc.description.abstractPrimary open-angle glaucoma (POAG) is a leading cause of blindness in the world. A primary risk factor for POAG is elevated intraocular pressure (IOP), caused by increased aqueous humor outflow resistance. Currently, lowering the IOP is the only effective way of treating glaucoma; however, the cause of increased outflow resistance remains unclear. This thesis will present a series of studies which investigated structures of the trabecular outflow pathway, including Schlemm’s canal endothelium, juxtacanalicular tissue, and trabecular beams, and their roles in regulating aqueous outflow resistance. The studies were conducted in both human and animal models using ex vivo ocular perfusion as well as in vitro microfluidic systems. In the first study, we investigated the effects of Y27632, a derivative of Rho-kinase inhibitor that is being developed as next generation glaucoma drug with unclear IOP lowering mechanism, on aqueous humor outflow dynamics and associated morphological changes in normal human eyes and laser-induced ocular hypertensive monkey eyes. In the second study, we developed and validated a novel three-dimensional microfluidic system using lymphatic microvascular endothelial cells. The microfluidic system can be used to study Schlemm’s canal endothelial cell dynamics and aqueous humor transport mechanism in the future. In the last study, we characterized the morphological structure, distribution, and thickness of the endothelial glycocalyx in the aqueous humor outflow pathway of human and bovine eyes. Together these studies will help define new directions for therapy that will help control IOP and preserve vision throughout a normal life span.en_US
dc.language.isoen_US
dc.rightsAttribution 4.0 Internationalen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectOphthalmologyen_US
dc.subjectGlaucomaen_US
dc.subjectMicrofluidicsen_US
dc.subjectSchlemm's canal endothelial cellsen_US
dc.subjectOutflow resistanceen_US
dc.subjectRho kinase inhibitoren_US
dc.subjectTrabecular meshworken_US
dc.titleStructural and functional investigation of the trabecular outflow pathwayen_US
dc.typeThesis/Dissertationen_US
dc.date.updated2016-06-15T22:36:46Z
etd.degree.nameDoctor of Philosophyen_US
etd.degree.leveldoctoralen_US
etd.degree.disciplineAnatomy & Neurobiologyen_US
etd.degree.grantorBoston Universityen_US


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International