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dc.contributor.authorPauli, Martinaen_US
dc.contributor.authorChakarov, Naydenen_US
dc.contributor.authorRupp, Oliveren_US
dc.contributor.authorKalinowski, Jörnen_US
dc.contributor.authorGoesmann, Alexanderen_US
dc.contributor.authorKrüger, Oliveren_US
dc.contributor.authorHoffman, Joseph Ivanen_US
dc.contributor.authorSorenson, Michael D.en_US
dc.coverage.spatialEnglanden_US
dc.date.accessioned2017-02-01T17:00:36Z
dc.date.available2017-02-01T17:00:36Z
dc.date.issued2015-12-09
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/26645667
dc.identifier.citationMartina Pauli, Nayden Chakarov, Oliver Rupp, Jörn Kalinowski, Alexander Goesmann, Michael D Sorenson, Oliver Krüger, Joseph Ivan Hoffman. 2015. "De novo assembly of the dual transcriptomes of a polymorphic raptor species and its malarial parasite.." BMC Genomics, Volume 16, pp. 1038 - ?. doi:10.1186/s12864-015-2254-1
dc.identifier.issn1471-2164
dc.identifier.urihttps://hdl.handle.net/2144/20249
dc.description.abstractBACKGROUND: Studies of non-model species are important for understanding the molecular processes underpinning phenotypic variation under natural ecological conditions. The common buzzard (Buteo buteo; Aves: Accipitriformes) is a widespread and common Eurasian raptor with three distinct plumage morphs that differ in several fitness-related traits, including parasite infestation. To provide a genomic resource for plumage polymorphic birds in general and to search for candidate genes relating to fitness, we generated a transcriptome from a single dead buzzard specimen plus easily accessible, minimally invasive samples from live chicks. RESULTS: We not only de novo assembled a near-complete buzzard transcriptome, but also obtained a significant fraction of the transcriptome of its malaria-like parasite, Leucocytozoon buteonis. By identifying melanogenesis-related transcripts that are differentially expressed in light ventral and dark dorsal feathers, but which are also expressed in other regions of the body, we also identified a suite of candidate genes that could be associated with fitness differences among the morphs. These include several immune-related genes, providing a plausible link between melanisation and parasite load. qPCR analysis of a subset of these genes revealed significant differences between ventral and dorsal feathers and an additional effect of morph. CONCLUSION: This new resource provides preliminary insights into genes that could be involved in fitness differences between the buzzard colour morphs, and should facilitate future studies of raptors and their malaria-like parasites.en_US
dc.format.extent1038 - ?en_US
dc.languageeng
dc.language.isoen_US
dc.relation.ispartofBMC Genomics
dc.rightsAttribution 4.0 Internationalen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAnimalsen_US
dc.subjectGenomicsen_US
dc.subjectHaemosporidaen_US
dc.subjectMelaninsen_US
dc.subjectRaptorsen_US
dc.subjectTranscriptomeen_US
dc.subjectNon-model organismen_US
dc.subjectCommon buzzard Buteo buteoen_US
dc.subjectPlasmodiumen_US
dc.subjectMelanin pathwayen_US
dc.subjectMolecular markersen_US
dc.subjectBioinformaticsen_US
dc.subjectAmino acid sequenceen_US
dc.subjectComputational biologyen_US
dc.subjectGene expression regulationen_US
dc.subjectGenetic markersen_US
dc.subjectHigh-throughput nucleotide sequencingen_US
dc.subjectMetabolic networks and pathwaysen_US
dc.subjectMolecular sequence annotationen_US
dc.subjectMolecular sequence dataen_US
dc.subjectPolymorphism, geneticen_US
dc.subjectReproducibility of resultsen_US
dc.subjectSequence alignmenten_US
dc.titleDe novo assembly of the dual transcriptomes of a polymorphic raptor species and its malarial parasiteen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/s12864-015-2254-1
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Arts & Sciencesen_US
pubs.organisational-groupBoston University, College of Arts & Sciences, Department of Biologyen_US
dc.identifier.orcid0000-0001-5375-2917 (Sorenson, Michael D)


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International