An immunological comparison of animal-grown and broth-grown shigella flexneri types lb and 3
Miller, John Tyle-
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It has been observed by many investigators that most persons seemed to possess an increased resistance to bacillary dysentery following recovery from this disease. However, shigella vaccines made from in vitro-grown organisms did not increase resistance to dysentery. The purpose of the present investigation was to determine whether or not Shigella flexneri produced antigens in the in vivo environment formed by culture-grown organisms which might resistance to reinfection. Immunological comparison of animal- and broth-grown Shigella flexneri types 1b and 3 involved comparing the agglutination reactions of animal- and broth-grown organisms and comparing their antigenic structures by means of the agar-diffusion precipitin technique. Antisera were prepared in rabbits by immunisation with either acetone-killed broth-grown organisms or viable organisms grown intra-abdominally in mice for three serial passages without culture. Antisera were absorbed with broth-grown Shigella flexneri of various types. Animal-grown Shigella flexneri were prepared by inoculating mice and guinea pigs with organisms suspended in neutralized streile 5 per cent mucin solution. The organisms were recovered by injecting intra-abdominally 0.15 M sodium chloride and aspirating the organisms with a needle and syringe. The resulting organisms were identified both serologically and culturally. Guinea pig-grown organisms were used for test antigens, whereas the mouse-grown organisms were used only for antiserum production in order to eliminate the possibility of reactions between normal guinea pig protein and antibody to mouse proteins. Saline solutions of water extracts of acetone-dried organisms were employed for the agar-diffusion precipitate stdies. [TRUNCATED]
Thesis (Ph.D.)--Boston University. Bibliography: p. 116-125.