Role of GSK-3 alpha beta in B cell proliferation during germinal center information

Abstract

Glycogen Synthase Kinase-3αß is an enzyme that is involved in cell cycle regulation by promoting the degradation of cyclin D1 and cycling D3 in cells. Special emphasis is placed in its regulatory role in B cells, as there it is evidence that suggests that this protein is inhibited during germinal center formation, where B cells undergo proliferation, somatic hypermutation and class switch recombination. By inducing DNA recombination via the Cre/lLxP recombination system and utilizing tamoxifen as a Cre activity inducer, B cells were culture in 40LB cells to form induced germinal center in vitro. Flow cytometry analysis suggests that in the absence of GSK-3 αß B cells proliferate extensively in germinal centers and being the process of class switch recombination. Although the results of this study are in accord with current theory, more experiments and research need to be made to validate the conclusions set forth in this study.