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dc.contributor.authorPastore, Edward Josephen_US
dc.date.accessioned2017-06-20T19:13:01Z
dc.date.available2017-06-20T19:13:01Z
dc.date.issued1959
dc.date.submitted1959
dc.identifier.otherb14673216
dc.identifier.urihttps://hdl.handle.net/2144/22492
dc.descriptionThesis (Ph.D.)--Boston Universityen_US
dc.description.abstractThe purpose of this investigation was to study the metabolism of normal human leukocytes. Leukocytes were incubated in vitro with c14-labeled acetate, and the fate of the radioactive carbon was determined by fractionation and analysis of the major, cell components. Leukocytes were obtained from whole blood by fibrinogen sedimentation and differential centrifugation. Optimal conditions for isolation and incubation of viable cells were developed and assessed using phase microscopy for direct observation of their morphological integrity and by their oxidative metabolism. Control experiments to determine the possible effect of erythrocyte utilization of acetate were run using twice the number of cells ordinarily found in leukocyte suspensions. No utilization of acetate by red blood cells was observed. Respiration studies were performed using standard Warburg manometry. Otherwise, incubations were carried out in modified Erlenmeyer flasks equipped with center wells for C02 collection and stoppered with serum bottle caps. Flasks were equilibrated and various additions were made to the suspensions using hypodermic needles. Total cells per flask varied from 1 to 5 X 108 with concentrations ranging from 60 to 80 X 106 cells per ml. of suspension. Response of respiration and combustion of acetate were directly proportional to cell number and no detrimental effects due to cell crowding were detectable within this range. [TRUNCATED]en_US
dc.language.isoen_US
dc.publisherBoston Universityen_US
dc.rightsBased on investigation of the BU Libraries' staff, this work is free of known copyright restrictions.en_US
dc.subjectHuman leukocyte antigenen_US
dc.subjectMetabolismen_US
dc.titleMetabolism of acetate by human leukocytesen_US
dc.typeThesis/Dissertationen_US
etd.degree.nameDoctor of Philosophyen_US
etd.degree.leveldoctoralen_US
etd.degree.disciplineBiochemistryen_US
etd.degree.grantorBoston Universityen_US


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