Methods of extracting antigens from the enteric bacteria
McNichol, Mary A.
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This investigation was conducted in order to determine the most efficient manner in which the "enteric bacteria" could be released for testing by the Ouchterlony technic. These results may prove useful in comparing the agar-gel precipitation technic with the usual agglutination technic for the typing of enteric bacteria. The organisms used were S. typhosa, strain Ty2; S. typhosa, strain 0-901; Sh. flexneri, type lb; Sh. flexneri, type 3; E. coli; Proteus species; and S. paratyphi B. Six different types of antigenic extract were prepared from these organisms when agar-grown. These extracts were saline, heated saline, trypsin, trichloroacetic acid, alumina, and sonic extracts. Concentrations of the antigenic extracts ranged from 0.1 to 50 mg/ml. Aging of the antigenic extracts for periods of up to and including three weeks was tried. Whole cultures, either living or after killing with phenol, merthiolate, formalin or heat, were tested. Antisera were prepared by immunizing rabbits with saline suspensions of acetone-dried organisms [TRUNCATED]
Thesis (M.A.)--Boston University