Transfer of fertilized ova in the hamster
Reamer, Gail R.
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Transfer of fertilized ova from a donor female to a uterine foster mother can be a valuable tool for attack of problems of gen etics, cancer, embryologJ, growth, immunology, and fertilization. The technique has been practiced in the rabbit, mouse, rat, and sheep with varying degrees of success. Holding media for the ova usually consist of blood serum, alone, or modified with a balanced saline solution. Diffusion chambers constructed of Millinore Filter membrane have proved valuable for 'in vivo' and 'in vitro' tissue culture and transnlantation. These chambers serve the useful function of mechanically isolating the contents from surrounding tissues and free cells, while providing diffusion of metabolites and oxygen into the chamber to nourish the enclosed tissue . Experimental procedure Donor albino females were mated with albino males. Approximately 24-33 hours after fertilization, when the ova were in the two cell stage, salpingectomies were performed. The ova were removed from the uterine tubes, and transferred to the ovarian bursa of pseudopregnant albino recipients [TRUNCATED]
Thesis (M.A.)--Boston University