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dc.contributor.authorTian, Leien_US
dc.contributor.authorSadras, Nitinen_US
dc.contributor.authorWaller, Lauraen_US
dc.contributor.authorPhillips, Zachary F.en_US
dc.contributor.authorD'Ambrosio, Michael V.en_US
dc.contributor.authorRulison, Jared J.en_US
dc.contributor.authorPatel, Hurshal S.en_US
dc.contributor.authorGande, Aditya V.en_US
dc.contributor.authorSwitz, Neil A.en_US
dc.contributor.authorFletcher, Daniel A.en_US
dc.coverage.spatialUnited Statesen_US
dc.date2015-03-19
dc.date.accessioned2017-11-16T18:51:29Z
dc.date.available2017-11-16T18:51:29Z
dc.date.issued2015
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/25969980
dc.identifier.citationZachary F. Phillips, Michael V. D'Ambrosio, Lei Tian, Jared J. Rulison, Hurshal S. Patel, Nitin Sadras, Aditya V. Gande, Neil A. Switz, Daniel A. Fletcher, Laura Waller. 2015. "Multi-contrast imaging and digital refocusing on a mobile microscope with a domed LED array.." PLoS One, v. 10, Issue 5, pp. e0124938 1-13.
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/2144/25611
dc.description.abstractWe demonstrate the design and application of an add-on device for improving the diagnostic and research capabilities of CellScope--a low-cost, smartphone-based point-of-care microscope. We replace the single LED illumination of the original CellScope with a programmable domed LED array. By leveraging recent advances in computational illumination, this new device enables simultaneous multi-contrast imaging with brightfield, darkfield, and phase imaging modes. Further, we scan through illumination angles to capture lightfield datasets, which can be used to recover 3D intensity and phase images without any hardware changes. This digital refocusing procedure can be used for either 3D imaging or software-only focus correction, reducing the need for precise mechanical focusing during field experiments. All acquisition and processing is performed on the mobile phone and controlled through a smartphone application, making the computational microscope compact and portable. Using multiple samples and different objective magnifications, we demonstrate that the performance of our device is comparable to that of a commercial microscope. This unique device platform extends the field imaging capabilities of CellScope, opening up new clinical and research possibilities.en_US
dc.format.extentp. 1-13en_US
dc.languageeng
dc.language.isoen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS One
dc.rightsAttribution 4.0 Internationalen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHumansen_US
dc.subjectMicroscopyen_US
dc.subjectSmartphoneen_US
dc.subjectSoftwareen_US
dc.subjectScience & technologyen_US
dc.subjectMultidisciplinary sciencesen_US
dc.subjectDifferential phase-contrasten_US
dc.subjectHigh-resolutionen_US
dc.subjectIlluminationen_US
dc.subjectFielden_US
dc.subjectDynamicsen_US
dc.subjectGrowthen_US
dc.subjectEquipment designen_US
dc.subjectFourier analysisen_US
dc.subjectImage processing, computer-assisteden_US
dc.subjectImaging, three-dimensionalen_US
dc.subjectPoint-of-care systemsen_US
dc.titleMulti-contrast imaging and digital refocusing on a mobile microscope with a domed LED arrayen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0124938
pubs.elements-sourcepubmeden_US
pubs.notesEmbargo: No embargoen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Engineeringen_US
pubs.organisational-groupBoston University, College of Engineering, Department of Electrical & Computer Engineeringen_US
pubs.publication-statusPublished onlineen_US
dc.identifier.orcid0000-0002-1316-4456 (Tian, Lei)


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International