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dc.contributor.authorSomkuwar, Sucharita S.
dc.contributor.authorKantak, Kathleen M.
dc.contributor.authorDwoskin, Linda P.
dc.coverage.spatialNetherlandsen_US
dc.date2015-02-03
dc.date.accessioned2017-11-20T19:28:37Z
dc.date.available2017-11-20T19:28:37Z
dc.date.issued2015-08-30
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/25680322
dc.identifier.citationSucharita S. Somkuwar, Kathleen M. Kantak, Linda P. Dwoskin. 2015. "Effect of methylphenidate treatment during adolescence on norepinephrine transporter function in orbitofrontal cortex in a rat model of attention deficit hyperactivity disorder.." J Neurosci Methods, v. 252, pp. 55 - 63.
dc.identifier.issn1872-678X
dc.identifier.urihttps://hdl.handle.net/2144/25616
dc.description.abstractAttention deficit hyperactivity disorder (ADHD) is associated with hypofunctional medial prefrontal cortex (mPFC) and orbitofrontal cortex (OFC). Methylphenidate (MPH) remediates ADHD, in part, by inhibiting the norepinephrine transporter (NET). MPH also reduces ADHD-like symptoms in spontaneously hypertensive rats (SHRs), a model of ADHD. However, effects of chronic MPH treatment on NET function in mPFC and OFC in SHR have not been reported. In the current study, long-term effects of repeated treatment with a therapeutically relevant oral dose of MPH during adolescence on NET function in subregions of mPFC (cingulate gyrus, prelimbic cortex and infralimbic cortex) and in the OFC of adult SHR, Wistar-Kyoto (WKY, inbred control) and Wistar (WIS, outbred control) rats were determined using in vivo voltammetry. Following local ejection of norepinephrine (NE), uptake rate was determined as peak amplitude (Amax)× first-order rate constant (k-1). In mPFC subregions, no strain or treatment effects were found in NE uptake rate. In OFC, NE uptake rate in vehicle-treated adult SHR was greater than in adult WKY and WIS administered vehicle. MPH treatment during adolescence normalized NE uptake rate in OFC in SHR. Thus, the current study implicates increased NET function in OFC as an underlying mechanism for reduced noradrenergic transmission in OFC, and consequently, the behavioral deficits associated with ADHD. MPH treatment during adolescence normalized NET function in OFC in adulthood, suggesting that the therapeutic action of MPH persists long after treatment cessation and may contribute to lasting reductions in deficits associated with ADHD.en_US
dc.description.sponsorshipUL1 TR000117 - NCATS NIH HHS; R01 DA011716 - NIDA NIH HHS; P50 DA005312 - NIDA NIH HHS; P50 DA05312 - NIDA NIH HHS; R01 DA11716 - NIDA NIH HHSen_US
dc.format.extentp. 55-63en_US
dc.languageengen_US
dc.language.isoen_USen_US
dc.publisherElsevier
dc.relation.ispartofJ Neurosci Methodsen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectNorepinephrine transporteren_US
dc.subjectIn vivo voltammetryen_US
dc.subjectSpontaneously hypertensive raten_US
dc.subjectAttention deficit/hyperactivity disorderen_US
dc.subjectOrbitofrontal cortexen_US
dc.subjectIn vivo voltammetryen_US
dc.subjectNorepinephrine transporteren_US
dc.subjectAnimalsen_US
dc.subjectElectrochemistryen_US
dc.subjectMethylphenidateen_US
dc.subjectNorepinephrineen_US
dc.subjectPiperazinesen_US
dc.subjectRats, Sprague-Dawleyen_US
dc.subjectElectrochemistryen_US
dc.subjectFemaleen_US
dc.subjectMaleen_US
dc.subjectNorepinephrine plasma membrane transport proteinsen_US
dc.subjectNorepinephrine transporteren_US
dc.subjectIn vivo voltammetryen_US
dc.subjectSpontaneously hypertensive raten_US
dc.subjectAttention deficit/hyperactivity disorderen_US
dc.subjectOrbitofrontal cortexen_US
dc.subjectScience & technologyen_US
dc.subjectLife sciences & biomedicineen_US
dc.subjectBiochemical research methodsen_US
dc.subjectBiochemistry & molecular biologyen_US
dc.subjectNeurosciences & neurologyen_US
dc.subjectSpontaneously hypertensive-raten_US
dc.subjectWistar-Kyoto raten_US
dc.subjectMedial prefrontal cortexen_US
dc.subjectImpulsive behavior scaleen_US
dc.subjectDeficit/hyperactivity disorderen_US
dc.subjectNucleus-accumbensen_US
dc.subjectDopamine uptakeen_US
dc.subjectAnimal-modelen_US
dc.subjectCortical alpha(2)-adrenoceptorsen_US
dc.subjectAge factorsen_US
dc.subjectAttention deficit disorder with hyperactivityen_US
dc.subjectAnalysis of varianceen_US
dc.subjectConditioning, classicalen_US
dc.subjectDisease models, animalen_US
dc.subjectRats, inbred SHRen_US
dc.subjectRats, inbred WKYen_US
dc.subjectRats, wistaren_US
dc.titleEffect of methylphenidate treatment during adolescence on norepinephrine transporter function in orbitofrontal cortex in a rat model of attention deficit hyperactivity disorderen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.jneumeth.2015.02.002
pubs.elements-sourcepubmeden_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Arts & Sciencesen_US
pubs.organisational-groupBoston University, College of Arts & Sciences, Department of Psychological & Brain Sciencesen_US
pubs.publication-statusPublisheden_US
dc.identifier.orcid0000-0003-1866-9485 (Kantak, Kathleen M)


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Attribution-NonCommercial-NoDerivatives 4.0 International
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