Release of histamine from isolated mast cells by compound 48/80 and specific antigen
Arnold, Mary E.
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Histamine liberation has long been associated with mast cells both in anaphylaxis and chemically induced histamine release. Although the work on intact animals and in vitro preparations did much to clarify conditions under which histamine is released, these preparations were not ideal. A proper experimental design would be one in which the mast cells were isolated from other tissue influences. With this in mind, the present investigations were carried out. Mast cells were isolated from the peritoneal fluid of the rat and aivided into aliquots. In this preparation of morphologically and physiologically intact mast cells, the effects of specific antigen and compound 48/80 were studied on isolated cells in an attempt to learn more about the mechanism of histamine release from the mast cell. [TRUNCATED]
Thesis (M.A.)--Boston University