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dc.contributor.authorSevenler, Derinen_US
dc.date.accessioned2018-02-13T16:43:19Z
dc.date.available2018-02-13T16:43:19Z
dc.date.issued2017
dc.identifier.urihttps://hdl.handle.net/2144/27008
dc.description.abstractThis dissertation describes a new type of molecular assay for nucleic acids and proteins. We call this technique a digital microarray since it is conceptually similar to conventional fluorescence microarrays, yet it performs enumerative (‘digital’) counting of the number captured molecules. Digital microarrays are approximately 10,000-fold more sensitive than fluorescence microarrays, yet maintain all of the strengths of the platform including low cost and high multiplexing (i.e., many different tests on the same sample simultaneously). Digital microarrays use gold nanorods to label the captured target molecules. Each gold nanorod on the array is individually detected based on its light scattering, with an interferometric microscopy technique called SP-IRIS. Our optimized high-throughput version of SP-IRIS is able to scan a typical array of 500 spots in less than 10 minutes. Digital DNA microarrays may have utility in applications where sequencing is prohibitively expensive or slow. As an example, we describe a digital microarray assay for gene expression markers of bacterial drug resistance.en_US
dc.language.isoen_US
dc.rightsAttribution-ShareAlike 4.0 Internationalen_US
dc.rights.urihttps://creativecommons.org/licenses/by-sa/4.0/
dc.subjectBiomedical engineeringen_US
dc.subjectBiosensoren_US
dc.subjectImmunoassayen_US
dc.subjectNanophotonicsen_US
dc.subjectNanotechnologyen_US
dc.subjectPlasmonicsen_US
dc.titleDevelopment of a digital microarray with interferometric reflectance imagingen_US
dc.typeThesis/Dissertationen_US
dc.date.updated2017-11-02T22:14:45Z
etd.degree.nameDoctor of Philosophyen_US
etd.degree.leveldoctoralen_US
etd.degree.disciplineBiomedical Engineeringen_US
etd.degree.grantorBoston Universityen_US


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Except where otherwise noted, this item's license is described as Attribution-ShareAlike 4.0 International