Comparative action of insulin and insulin-like serum factors on rat striated muscle and epididymal adipose tissue

Abstract

This investigation was conducted in order to compare both in vitro and in vivo the differences in rat striated muscle and adipose tissue metabolism as influenced by insulin and insulin-like serum factors. For in vitro studies, the decrease in medium glucose concentration compared to basal tissue activity was used as an assessment of insulin activity, using both epididymal adipose tissue and hemidiaphragm from the same rat. In vivo studies compared the incorporation of parenterally administered U-c14-glucose into glycogen of striated muscle and into glycogen and fatty acids in adipose tissue in presence and absence of insulin. It proved of particular interest to compare the influence of intraperitoneally and intravenously injected insulin [TRUNCATED]