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dc.contributor.authorMillien, Guetchynen_US
dc.contributor.authorSpira, Avrumen_US
dc.contributor.authorHinds, Anneen_US
dc.contributor.authorWang, Junlingen_US
dc.contributor.authorWilliams, Mary C.en_US
dc.contributor.authorRamirez, Maria I.en_US
dc.date.accessioned2012-01-09T20:53:13Z
dc.date.available2012-01-09T20:53:13Z
dc.date.copyright2006
dc.date.issued2006-7-25
dc.identifier.citationMillien, Guetchyn, Avrum Spira, Anne Hinds, Junling Wang, Mary C Williams, Maria I Ramirez. "Alterations in gene expression in T1α null lung: a model of deficient alveolar sac development" BMC Developmental Biology 6:35. (2006)
dc.identifier.issn1471-213X
dc.identifier.urihttps://hdl.handle.net/2144/2886
dc.description.abstractBACKGROUND. Development of lung alveolar sacs of normal structure and size at late gestation is necessary for the gas exchange process that sustains respiration at birth. Mice lacking the lung differentiation gene T1α [T1α(-/-)] fail to form expanded alveolar sacs, resulting in respiratory failure at birth. Since little is known about the molecular pathways driving alveolar sacculation, we used expression microarrays to identify genes altered in the abnormal lungs and, by inference, may play roles in normal lung morphogenesis. RESULTS. Altered expression of genes related to cell-cell interaction, such as ephrinA3, are observed in T1α(-/-) at E18.5. At term, FosB, Egr1, MPK-1 and Nur77, which can function as negative regulators of the cell-cycle, are down-regulated. This is consistent with the hyperproliferation of peripheral lung cells in term T1α (-/-) lungs reported earlier. Biochemical assays show that neither PCNA nor p21 are altered at E18.5. At term in contrast, PCNA is increased, and p21 is decreased. CONCLUSION. This global analysis has identified a number of candidate genes that are significantly altered in lungs in which sacculation is abnormal. Many genes identified were not previously associated with lung development and may participate in formation of alveolar sacs prenatally.en_US
dc.description.sponsorshipNational Heart, Lung, and Blood Institute (Program Project Grant HL47049); Doris Duke Charitable Foundation (Clinical Scientist Development Award)en_US
dc.language.isoen
dc.publisherBioMed Centralen_US
dc.rightsCopyright 2006 Millien et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.0
dc.titleAlterations in gene expression in T1α null lung: a model of deficient alveolar sac developmenten_US
dc.typeArticleen_US
dc.identifier.doi10.1186/1471-213X-6-35
dc.identifier.pmid16869965
dc.identifier.pmcid1562362


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Copyright 2006 Millien et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as Copyright 2006 Millien et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.