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dc.contributor.authorYang, Ruohuien_US
dc.contributor.authorWeber, Timothy D.en_US
dc.contributor.authorWitkowski, Ellen D.en_US
dc.contributor.authorDavison, Ian G.en_US
dc.contributor.authorMertz, Jeromeen_US
dc.coverage.spatialEnglanden_US
dc.date2017-06-07
dc.date.accessioned2018-05-14T17:42:51Z
dc.date.available2018-05-14T17:42:51Z
dc.date.issued2017-07-19
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/28725020
dc.identifier.citationRuohui Yang, Timothy D Weber, Ellen D Witkowski, Ian G Davison, Jerome Mertz. 2017. "Neuronal imaging with ultrahigh dynamic range multiphoton microscopy.." Sci Rep, Volume 7, Issue 1:5817.
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/2144/28914
dc.description.abstractMultiphoton microscopes are hampered by limited dynamic range, preventing weak sample features from being detected in the presence of strong features, or preventing the capture of unpredictable bursts in sample strength. We present a digital electronic add-on technique that vastly improves the dynamic range of a multiphoton microscope while limiting potential photodamage. The add-on provides real-time negative feedback to regulate the laser power delivered to the sample, and a log representation of the sample strength to accommodate ultrahigh dynamic range without loss of information. No microscope hardware modifications are required, making the technique readily compatible with commercial instruments. Benefits are shown in both structural and in-vivo functional mouse brain imaging applications.en_US
dc.description.sponsorshipR21 EY027549 - NEI NIH HHSen_US
dc.format.extent5817en_US
dc.languageeng
dc.relation.ispartofSci Rep
dc.rightsOpen Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectScience & technologyen_US
dc.subjectLight exposure microscopyen_US
dc.subjectNeural activityen_US
dc.subjectPhototoxicityen_US
dc.subjectIlluminationen_US
dc.subjectMiceen_US
dc.titleNeuronal imaging with ultrahigh dynamic range multiphoton microscopyen_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41598-017-06065-7
pubs.elements-sourcepubmeden_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Arts & Sciencesen_US
pubs.organisational-groupBoston University, College of Arts & Sciences, Department of Biologyen_US
pubs.organisational-groupBoston University, College of Engineeringen_US
pubs.organisational-groupBoston University, College of Engineering, Department of Biomedical Engineeringen_US
pubs.publication-statusPublished onlineen_US


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Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Except where otherwise noted, this item's license is described as Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.