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dc.contributor.authorSharma, Ruchiraen_US
dc.contributor.authorIshimaru, Yoshiroen_US
dc.contributor.authorDavison, Ianen_US
dc.contributor.authorIkegami, Kentaroen_US
dc.contributor.authorChien, Ming-Shanen_US
dc.contributor.authorYou, Helenaen_US
dc.contributor.authorChi, Quiyien_US
dc.contributor.authorKubota, Momokaen_US
dc.contributor.authorYohda, Masafumien_US
dc.contributor.authorEhlers, Michaelen_US
dc.contributor.authorMatsunami, Hiroakien_US
dc.coverage.spatialEnglanden_US
dc.date2017-02-16
dc.date.accessioned2018-05-14T18:32:02Z
dc.date.available2018-05-14T18:32:02Z
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/28262096
dc.identifier.citationRuchira Sharma, Yoshiro Ishimaru, Ian Davison, Kentaro Ikegami, Ming-Shan Chien, Helena You, Quiyi Chi, Momoka Kubota, Masafumi Yohda, Michael Ehlers, Hiroaki Matsunami. "Olfactory receptor accessory proteins play crucial roles in receptor function and gene choice.." eLife 2017;6:e21895 DOI: 10.7554/eLife.21895
dc.identifier.issn2050-084X
dc.identifier.urihttps://hdl.handle.net/2144/28917
dc.description.abstractEach of the olfactory sensory neurons (OSNs) chooses to express a single G protein-coupled olfactory receptor (OR) from a pool of hundreds. Here, we show the receptor transporting protein (RTP) family members play a dual role in both normal OR trafficking and determining OR gene choice probabilities. Rtp1 and Rtp2 double knockout mice (RTP1,2DKO) show OR trafficking defects and decreased OSN activation. Surprisingly, we discovered a small subset of the ORs are expressed in larger numbers of OSNs despite the presence of fewer total OSNs in RTP1,2DKO. Unlike typical ORs, some overrepresented ORs show robust cell surface expression in heterologous cells without the co-expression of RTPs. We present a model in which developing OSNs exhibit unstable OR expression until they choose to express an OR that exits the ER or undergo cell death. Our study sheds light on the new link between OR protein trafficking and OR transcriptional regulation.en_US
dc.description.sponsorshipR01 DC012095 - NIDCD NIH HHS; R01 DC014423 - NIDCD NIH HHSen_US
dc.languageeng
dc.relation.ispartofeLife
dc.rightsCopyright © 2017, Sharma et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectScience & technologyen_US
dc.subjectLife sciences & biomedicineen_US
dc.subjectBiologyen_US
dc.subjectMammalian odorant receptorsen_US
dc.subjectSensory neuronsen_US
dc.subjectGPCRen_US
dc.subjectUPRen_US
dc.subjectCell biologyen_US
dc.subjectChaperoneen_US
dc.subjectEpigeneticen_US
dc.subjectGene regulationen_US
dc.subjectMouseen_US
dc.subjectNeuroscienceen_US
dc.subjectOdoranten_US
dc.subjectOlfactionen_US
dc.subjectUnfolded protein responseen_US
dc.subjectAnimalsen_US
dc.subjectCarrier proteinsen_US
dc.subjectGene expression regulationen_US
dc.subjectMice, knockouten_US
dc.subjectModels, biologicalen_US
dc.subjectReceptors, odoranten_US
dc.subjectAnimalsen_US
dc.subjectCarrier proteinsen_US
dc.titleOlfactory receptor accessory proteins play crucial roles in receptor function and gene choiceen_US
dc.typeArticleen_US
dc.identifier.doi10.7554/eLife.21895
pubs.elements-sourcepubmeden_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Arts & Sciencesen_US
pubs.organisational-groupBoston University, College of Arts & Sciences, Department of Biologyen_US
pubs.publication-statusPublished onlineen_US


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Copyright © 2017, Sharma et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Except where otherwise noted, this item's license is described as Copyright © 2017, Sharma et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.