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dc.contributor.authorPiacentino, Michael L.en_US
dc.contributor.authorZuch, Daniel T.en_US
dc.contributor.authorFishman, Julieen_US
dc.contributor.authorRose, Sviatlanaen_US
dc.contributor.authorSperanza, Emily E.en_US
dc.contributor.authorLi, Christyen_US
dc.contributor.authorYu, Jiaen_US
dc.contributor.authorChung, Oliveren_US
dc.contributor.authorRamachandran, Jananien_US
dc.contributor.authorFerrell, Patricken_US
dc.contributor.authorPatel, Vijetaen_US
dc.contributor.authorReyna, Arleneen_US
dc.contributor.authorHameeduddin, Hajerahen_US
dc.contributor.authorChaves, Jamesen_US
dc.contributor.authorHewitt, Finnegan B.en_US
dc.contributor.authorBardot, Evanen_US
dc.contributor.authorLee, Daviden_US
dc.contributor.authorCore, Amanda B.en_US
dc.contributor.authorHogan, John D.en_US
dc.contributor.authorKeenan, Jessica Len_US
dc.contributor.authorLuo, Lingqien_US
dc.contributor.authorCoulombe-Huntington, Jasminen_US
dc.contributor.authorBlute, Todd A.en_US
dc.contributor.authorOleinik, Ekaterinaen_US
dc.contributor.authorIbn-Salem, Jonasen_US
dc.contributor.authorPoustka, Albert J.en_US
dc.contributor.authorBradham, Cynthia A.en_US
dc.coverage.spatialEnglanden_US
dc.date2016-01-05
dc.date.accessioned2018-05-17T19:28:00Z
dc.date.available2018-05-17T19:28:00Z
dc.date.issued2016-02-15
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/26755701
dc.identifier.citationMichael L Piacentino, Daniel T Zuch, Julie Fishman, Sviatlana Rose, Emily E Speranza, Christy Li, Jia Yu, Oliver Chung, Janani Ramachandran, Patrick Ferrell, Vijeta Patel, Arlene Reyna, Hajerah Hameeduddin, James Chaves, Finnegan B Hewitt, Evan Bardot, David Lee, Amanda B Core, John D Hogan, Jessica L Keenan, Lingqi Luo, Jasmin Coulombe-Huntington, Todd A Blute, Ekaterina Oleinik, Jonas Ibn-Salem, Albert J Poustka, Cynthia A Bradham. 2016. "RNA-Seq identifies SPGs as a ventral skeletal patterning cue in sea urchins.." Development, Volume 143, Issue 4, pp. 703 - 714.
dc.identifier.issn1477-9129
dc.identifier.urihttps://hdl.handle.net/2144/28956
dc.description.abstractThe sea urchin larval skeleton offers a simple model for formation of developmental patterns. The calcium carbonate skeleton is secreted by primary mesenchyme cells (PMCs) in response to largely unknown patterning cues expressed by the ectoderm. To discover novel ectodermal cues, we performed an unbiased RNA-Seq-based screen and functionally tested candidates; we thereby identified several novel skeletal patterning cues. Among these, we show that SLC26a2/7 is a ventrally expressed sulfate transporter that promotes a ventral accumulation of sulfated proteoglycans, which is required for ventral PMC positioning and skeletal patterning. We show that the effects of SLC perturbation are mimicked by manipulation of either external sulfate levels or proteoglycan sulfation. These results identify novel skeletal patterning genes and demonstrate that ventral proteoglycan sulfation serves as a positional cue for sea urchin skeletal patterning.en_US
dc.format.extentp. 703 - 714en_US
dc.languageeng
dc.relation.ispartofDevelopment
dc.rights© 2016. Published by The Company of Biologists Ltden_US
dc.subjectScience & technologyen_US
dc.subjectLife sciences & biomedicineen_US
dc.subjectDevelopmental biologyen_US
dc.subjectPatterningen_US
dc.subjectSkeletonen_US
dc.subjectSulfated proteoglycanen_US
dc.subjectRNA-Seqen_US
dc.subjectAnimalsen_US
dc.subjectBody patterningen_US
dc.subjectCation transport proteinsen_US
dc.subjectCell differentiationen_US
dc.subjectEctodermen_US
dc.subjectEmbryo, nonmammalianen_US
dc.subjectGene expression regulation, developmentalen_US
dc.subjectMesodermen_US
dc.subjectModels, biologicalen_US
dc.subjectNickelen_US
dc.subjectProteoglycansen_US
dc.subjectSea urchinsen_US
dc.subjectSequence analysis, RNAen_US
dc.subjectSignal transductionen_US
dc.subjectSulfatesen_US
dc.subjectVascular endothelial growth factor Aen_US
dc.subjectp38 mitogen-activated protein kinasesen_US
dc.subjectBiological sciencesen_US
dc.subjectMedical and health sciencesen_US
dc.titleRNA-Seq identifies SPGs as a ventral skeletal patterning cue in sea urchinsen_US
dc.typeArticleen_US
dc.identifier.doi10.1242/dev.129312
pubs.elements-sourcepubmeden_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Arts & Sciencesen_US
pubs.organisational-groupBoston University, College of Arts & Sciences, Department of Biologyen_US
pubs.publication-statusPublisheden_US


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