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dc.contributor.authorBaumann, Cory W.en_US
dc.contributor.authorLiu, Haiming M.en_US
dc.contributor.authorThompson, LaDora V.en_US
dc.coverage.spatialUnited Statesen_US
dc.date2016-07-26
dc.date.accessioned2018-05-24T18:43:53Z
dc.date.available2018-05-24T18:43:53Z
dc.date.issued2016
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/27513942
dc.identifier.citationCory W Baumann, Haiming M Liu, LaDora V Thompson. 2016. "Denervation-Induced Activation of the Ubiquitin-Proteasome System Reduces Skeletal Muscle Quantity Not Quality.." PLoS One, v. 11, issue 8
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/2144/29010
dc.description.abstractIt is well known that the ubiquitin-proteasome system is activated in response to skeletal muscle wasting and functions to degrade contractile proteins. The loss of these proteins inevitably reduces skeletal muscle size (i.e., quantity). However, it is currently unknown whether activation of this pathway also affects function by impairing the muscle's intrinsic ability to produce force (i.e., quality). Therefore, the purpose of this study was twofold, (1) document how the ubiquitin-proteasome system responds to denervation and (2) identify the physiological consequences of these changes. To induce soleus muscle atrophy, C57BL6 mice underwent tibial nerve transection of the left hindlimb for 7 or 14 days (n = 6-8 per group). At these time points, content of several proteins within the ubiquitin-proteasome system were determined via Western blot, while ex vivo whole muscle contractility was specifically analyzed at day 14. Denervation temporarily increased several key proteins within the ubiquitin-proteasome system, including the E3 ligase MuRF1 and the proteasome subunits 19S, α7 and β5. These changes were accompanied by reductions in absolute peak force and power, which were offset when expressed relative to physiological cross-sectional area. Contrary to peak force, absolute and relative forces at submaximal stimulation frequencies were significantly greater following 14 days of denervation. Taken together, these data represent two keys findings. First, activation of the ubiquitin-proteasome system is associated with reductions in skeletal muscle quantity rather than quality. Second, shortly after denervation, it appears the muscle remodels to compensate for the loss of neural activity via changes in Ca2+ handling.en_US
dc.description.sponsorshipT32 AG029796 - NIA NIH HHSen_US
dc.languageeng
dc.relation.ispartofPLoS One
dc.rightsAttribution 4.0 Internationalen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAnimalsen_US
dc.subjectMaleen_US
dc.subjectMiceen_US
dc.subjectUbiquitinen_US
dc.subjectMice, inbred C57BLen_US
dc.subjectMuscle contractionen_US
dc.subjectMuscle denervationen_US
dc.subjectMuscle, skeletalen_US
dc.subjectMuscular atrophyen_US
dc.subjectProteasome endopeptidase complexen_US
dc.subjectMD multidisciplinaryen_US
dc.subjectGeneral science & technologyen_US
dc.titleDenervation-induced activation of the ubiquitin-proteasome system reduces skeletal muscle quantity not qualityen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0160839
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/en_US
pubs.elements-sourcepubmeden_US
pubs.notesEmbargo: Not knownen_US
pubs.organisational-groupBoston Universityen_US
pubs.organisational-groupBoston University, College of Health & Rehabilitation Sciences: Sargent Collegeen_US
pubs.organisational-groupBoston University, College of Health & Rehabilitation Sciences: Sargent College, Physical Therapy and Athletic Trainingen_US
pubs.publication-statusPublished onlineen_US


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International