Biologically active substances affecting gonadotrophin-induced ovulation in immature rats
France, Evelyn Susan
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A number of biologically active compounds known to influence various aspects of mammalian reproduction were investigated in order to determine specifically whether or not they affect gonadotrophin-induced ovulation and, if so, whether they act directly on the ovaries by blockage of the utilization of gonadotrophins or indirectly via the hypothalamico-hypophyseal axis. Maximal ovulatory response of 35 +/- 5 ova were routinely induced in 21-day-old intact Wistar rats by the subcutaneous injection of 20 IU pregnant mare serum (PMS) followed by the intraperitioneal injection of 25 IU human chorionic gonadotrophin (HCG) 56 hours later. Animals were sacrificed 20 hours after HCG injection, and body weights were recorded. Fallopian tubes were removed, and ova within them were recovered and counted. Ovaries were weighed to the nearest 0.2 mg and weights expressed as actual wet weight and relative weight (mg/100 gm body weight). Average numbers of ova shed and ovarian weights of the gonadotrophin-treated control groups were compared with those of the experimental groups, which were also administered single doses of the biologically active compounds. If a given compound significantly inhibited the superovulatory response in intact rats, it was then administered under the same conditions to rats hypophysectomized at 25 days of age. [TRUNCATED]
Thesis (Ph.D.)--Boston University
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