An ES-Like Pluripotent State in FGF-Dependent Murine iPS cells
Di Stefano, Bruno
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Citation (published version)Di Stefano, Bruno, Christa Buecker, Federica Ungaro, Alessandro Prigione, Hsu-Hsin Chen, Maaike Welling, Maureen Eijpe, Gustavo Mostoslavsky, Paul Tesar, James Adjaye, Niels Geijsen, Vania Broccoli. "An ES-Like Pluripotent State in FGF-Dependent Murine iPS cells" PLoS ONE 5(12): e16092. (2010)
Recent data demonstrates that stem cells can exist in two morphologically, molecularly and functionally distinct pluripotent states; a naïve LIF-dependent pluripotent state which is represented by murine embryonic stem cells (mESCs) and an FGF-dependent primed pluripotent state represented by murine and rat epiblast stem cells (EpiSCs). We find that derivation of induced pluripotent stem cells (iPSCs) under EpiSC culture conditions yields FGF-dependent iPSCs from hereon called FGF-iPSCs) which, unexpectedly, display naïve ES-like/ICM properties. FGF-iPSCs display X-chromosome activation, multi-lineage differentiation, teratoma competence and chimera contribution in vivo. Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions. Characterization of the key molecular signalling pathways revealed FGF-iPSCs to depend on the Activin/Nodal and FGF pathways, while signalling through the JAK-STAT pathway is not required for FGF-iPS cell maintenance. Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions.
RightsDi Stefano et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- MED: Medicine Papers