Silk-fibronectin protein alloy fibres support cell adhesion and viability as a high strength, matrix fibre analogue
Jacobsen, Matthew M.
Gyune Rim, Nae
Smith, Michael L.
Wong, Joyce Y.
MetadataShow full item record
Citation (published version)Matthew M Jacobsen, David Li, Nae Gyune Rim, Daniel Backman, Michael L Smith, Joyce Y Wong. 2017. "Silk-fibronectin protein alloy fibres support cell adhesion and viability as a high strength, matrix fibre analogue.." Sci Rep, Volume 7: 45653.
Silk is a natural polymer with broad utility in biomedical applications because it exhibits general biocompatibility and high tensile material properties. While mechanical integrity is important for most biomaterial applications, proper function and integration also requires biomaterial incorporation into complex surrounding tissues for many physiologically relevant processes such as wound healing. In this study, we spin silk fibroin into a protein alloy fibre with whole fibronectin using wet spinning approaches in order to synergize their respective strength and cell interaction capabilities. Results demonstrate that silk fibroin alone is a poor adhesive surface for fibroblasts, endothelial cells, and vascular smooth muscle cells in the absence of serum. However, significantly improved cell attachment is observed to silk-fibronectin alloy fibres without serum present while not compromising the fibres' mechanical integrity. Additionally, cell viability is improved up to six fold on alloy fibres when serum is present while migration and spreading generally increase as well. These findings demonstrate the utility of composite protein alloys as inexpensive and effective means to create durable, biologically active biomaterials.
RightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/