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dc.contributor.authorKlein, Leroyen_US
dc.date.accessioned2018-07-31T13:48:53Z
dc.date.available2018-07-31T13:48:53Z
dc.date.issued1952
dc.date.submitted1952
dc.identifier.otherb14763709
dc.identifier.urihttps://hdl.handle.net/2144/30125
dc.descriptionThesis (M.A)--Boston Universityen_US
dc.description.abstractThe object of this problem was to demonstrate and discuss the kinetics of the bacteriolytic action of egg white lysozyme. This was done to elucidate further the enzymic nature and the mechanism of lysozyme action. The activity of the enzyme was measured by a turbidimetric method. Crystalline lysozyme was diluted to various concentrations with phosphate buffer (pH 6.2) and incubated at various temperatures with bacterial substrates such as Micrococcus lysodeikticus and Sarcina luten. The decrease in optical density was followed on a Colemen spectrophotometer, using a wave length of 540. The data indicates that the reaction follows the mono-molecular equation as others have earlier cited in the literature. The average Q10 value was 1.75 +/- 0.14. The energy of activation (u) was found to be 10,200 +/- 1600 cal for experiments conducted with lysozyme at three different concentrations and on both substrates. The similarity of "u" values indicates that the lyzing action of lysozyme is characteristic of the enzyme on both substrates. It appears that the lysozyme is splitting the same type of linkage in the mucopolysaccharide component of the bacterial cell membrane. Thus this enzymatic reaction is quite specific. It was also shown that the velocity of the lytic action by lysozyme is determined by the amount of lysozyme absorbed, i.e. - absorption is the rate-determining step.en_US
dc.language.isoen_US
dc.publisherBoston Universityen_US
dc.rightsBased on investigation of the BU Libraries' staff, this work is free of known copyright restrictions.en_US
dc.subjectLysozymeen_US
dc.subjectBacteriaen_US
dc.titleThe mechanism of the activity of lysozyme on bacteriaen_US
dc.typeThesis/Dissertationen_US
etd.degree.nameMaster of Artsen_US
etd.degree.levelmastersen_US
etd.degree.disciplineBiochemistryen_US
etd.degree.grantorBoston Universityen_US
dc.identifier.barcode11719025695034
dc.identifier.mmsid99175887570001161


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