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dc.contributor.advisorWong, Joyce Y.en_US
dc.contributor.authorRim, Nae Gyuneen_US
dc.date.accessioned2018-08-07T13:07:03Z
dc.date.available2018-08-07T13:07:03Z
dc.date.issued2018
dc.identifier.urihttps://hdl.handle.net/2144/30707
dc.description.abstractTo successfully develop a functional tissue-engineered vascular patch, recapitulating the hierarchical structure of vessel is critical to mimic mechanical properties. Here, we use a cell sheet engineering strategy with micropatterning technique to control structural organization of bovine aortic vascular smooth muscle cell (VSMC) sheets. Actin filament staining and image analysis showed clear cellular alignment of VSMC sheets cultured on patterned substrates. Viability of harvested VSMC sheets was confirmed by Live/Dead® cell viability assay after 24 and 48 hours of transfer. VSMC sheets stacked to generate bilayer VSMC patches exhibited strong inter-layer bonding as shown by lap shear test. Uniaxial tensile testing of monolayer VSMC sheets and bilayer VSMC patches displayed nonlinear, anisotropic stress-stretch response similar to the biomechanical characteristic of a native arterial wall. Collagen content and structure were characterized to determine the effects of patterning and stacking on extracellular matrix of VSMC sheets. Using finite-element modeling to simulate uniaxial tensile testing of bilayer VSMC patches, we found the stress-stretch response of bilayer patterned VSMC patches under uniaxial tension to be predicted using an anisotropic hyperelastic constitutive model. Thus, our cell sheet harvesting system combined with biomechanical modeling is a promising approach to generate building blocks for tissue-engineered vascular patches with structure and mechanical behavior mimicking native tissue.en_US
dc.language.isoen_US
dc.subjectBiomedical engineeringen_US
dc.subjectFinite element modelingen_US
dc.subjectHydrogelen_US
dc.subjectTensile testingen_US
dc.subjectVascular smooth muscle cellen_US
dc.titleMicropatterned cell sheets as structural building blocks for biomimetic vascular patch applicationen_US
dc.typeThesis/Dissertationen_US
dc.date.updated2018-07-03T01:03:53Z
etd.degree.nameDoctor of Philosophyen_US
etd.degree.leveldoctoralen_US
etd.degree.disciplineBiomedical Engineeringen_US
etd.degree.grantorBoston Universityen_US


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