Show simple item record

dc.contributor.authorOrtiz, Luisen_US
dc.contributor.authorLashkaripour, Alien_US
dc.contributor.authorDensmore, Douglasen_US
dc.date.accessioned2018-08-14T14:24:53Z
dc.date.available2018-08-14T14:24:53Z
dc.date.issued2018-08-03
dc.identifier.citationL. Ortiz, A. Lashkaripour, D. Densmore. (2018). Automating Functional Enzyme Screening & Characterization. In The Proceedings of the 10th International Workshop on Bio-Design Automation.
dc.identifier.urihttps://hdl.handle.net/2144/30782
dc.descriptionThis work has been presented in the 10th IWBDA workshop.en_US
dc.description.abstractMicrofluidics continue to gain traction as an inexpensive alternative to standard multi-well plate-based, and flow cytometry- based, assay platforms. These devices are especially useful for the types of ultra-high throughput screens needed for enzyme discovery applications where large numbers (>106) of unique samples must be screened rapidly1. Coupled with cell-free protein synthesis2, microfluidics are being used to identify novel enzymes useful for a variety of applications with unprecedented speed. However, these devices are typically produced using PDMS, and require considerable infrastructure and artisanal skill to fabricate, limiting their accessibility. Likewise, enzyme hits obtained from a screen are often validated manually and would benefit from automation of downstream validation processes. To address these limitations, we propose a workflow which leverages software tools to automate the rapid design and fabrication of low-cost polycarbonate microfluidic devices for use as high-throughput screening platforms for enzyme discovery, as well as an automated DNA assembly tool to streamline validation of screening candidates. Using this workflow, we aim to identify novel oxidoreductase enzymes from environmental metagenomic DNA libraries, for use in electrochemical biosensors.en_US
dc.language.isoen_US
dc.publisherInternational Workshop on Bio-Design Automation (IWBDA)en_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United Statesen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/
dc.subjectSynthetic biologyen_US
dc.subjectCell-freeen_US
dc.subjectScreeningen_US
dc.subjectMicrofluidicsen_US
dc.subjectCADen_US
dc.titleAutomating functional enzyme screening & characterizationen_US
dc.typeConference materialsen_US


This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States