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dc.contributor.authorSullivan, James C.en_US
dc.contributor.authorSher, Danielen_US
dc.contributor.authorEisenstein, Miriamen_US
dc.contributor.authorShigesada, Katsuyaen_US
dc.contributor.authorReitzel, Adam M.en_US
dc.contributor.authorMarlow, Heatheren_US
dc.contributor.authorLevanon, Ditsaen_US
dc.contributor.authorGroner, Yoramen_US
dc.contributor.authorFinnerty, John R.en_US
dc.contributor.authorGat, Urien_US
dc.date.accessioned2012-01-11T16:42:55Z
dc.date.available2012-01-11T16:42:55Z
dc.date.copyright2008
dc.date.issued2008-8-5
dc.identifier.citationSullivan, James C, Daniel Sher, Miriam Eisenstein, Katsuya Shigesada, Adam M Reitzel, Heather Marlow, Ditsa Levanon, Yoram Groner, John R Finnerty, Uri Gat. "The evolutionary origin of the Runx/CBFbeta transcription factors – Studies of the most basal metazoans" BMC Evolutionary Biology 8:228. (2008)
dc.identifier.issn1471-2148
dc.identifier.urihttps://hdl.handle.net/2144/3118
dc.description.abstractBACKGROUND. Members of the Runx family of transcriptional regulators, which bind DNA as heterodimers with CBFβ, are known to play critical roles in embryonic development in many triploblastic animals such as mammals and insects. They are known to regulate basic developmental processes such as cell fate determination and cellular potency in multiple stem-cell types, including the sensory nerve cell progenitors of ganglia in mammals. RESULTS. In this study, we detect and characterize the hitherto unexplored Runx/CBFβ genes of cnidarians and sponges, two basal animal lineages that are well known for their extensive regenerative capacity. Comparative structural modeling indicates that the Runx-CBFβ-DNA complex from most cnidarians and sponges is highly similar to that found in humans, with changes in the residues involved in Runx-CBFβ dimerization in either of the proteins mirrored by compensatory changes in the binding partner. In situ hybridization studies reveal that Nematostella Runx and CBFβ are expressed predominantly in small isolated foci at the base of the ectoderm of the tentacles in adult animals, possibly representing neurons or their progenitors. CONCLUSION. These results reveal that Runx and CBFβ likely functioned together to regulate transcription in the common ancestor of all metazoans, and the structure of the Runx-CBFβ-DNA complex has remained extremely conserved since the human-sponge divergence. The expression data suggest a hypothesis that these genes may have played a role in nerve cell differentiation or maintenance in the common ancestor of cnidarians and bilaterians.en_US
dc.description.sponsorshipNational Science Foundation (IBN-0212773, FP-91656101-0); Boston University SPRInG (20-202-8103-9); Israel Science Foundation (825/07)en_US
dc.language.isoen
dc.publisherBioMed Centralen_US
dc.rightsCopyright 2008 Sullivan et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.0
dc.titleThe Evolutionary Origin of the Runx/CBFbeta Transcription Factors – Studies of the Most Basal Metazoansen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/1471-2148-8-228
dc.identifier.pmid18681949
dc.identifier.pmcid2527000


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Copyright 2008 Sullivan et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as Copyright 2008 Sullivan et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.