Show simple item record

dc.contributor.authorKlausner, J. M.en_US
dc.contributor.authorAbu-Abid, S.en_US
dc.contributor.authorAlexander, J. S.en_US
dc.contributor.authorHanshke-Mineau, R.en_US
dc.contributor.authorGoldman, G.en_US
dc.contributor.authorMorel, N.en_US
dc.contributor.authorValeri, C. R.en_US
dc.contributor.authorShepro, D.en_US
dc.contributor.authorHechtman, H. B.en_US
dc.date.accessioned2012-01-11T16:44:59Z
dc.date.available2012-01-11T16:44:59Z
dc.date.copyright1994
dc.date.issued1994
dc.identifier.citationKlausner, J. M., S. Abu-Abid, J. S. Alexander, R. Hanshke-Mineau, G. Goldman, N. Morel, C. R. Valeri, D. Shepro, H. B. Hechtman. "Thromboxane Modulates Endothelial Permeability" Mediators of Inflammation 3(2): 149-153. (1994)
dc.identifier.issn1466-1861
dc.identifier.urihttps://hdl.handle.net/2144/3123
dc.description.abstractThe study tests the role of thromboxane in modulating microvascular permeability in vitro. Cultured monolayers of bovine aortic endothelial cells were challenged with the thromboxane (Tx) mimic U46619. This led to disassembly of actin microfilaments, cell rounding, border retraction and interendotheHal gap formation. Pretreatment with the Tx receptor antagonist SQ 29,548 prevented the Tx mimic-induced cytoskeletal changes. The Tx mimic also altered endothelial cell barrier function. Increased permeability was indicated by the increased passage of labelled albumin across monolayers cultured on microcarriers, relative to untreated endothelial cells (p < 0.05). Furthermore, electron microscopy of endothelial cells cultured on the basement membrane of human placental amnion indicated increased permeability based on wide, interendotheHal gap formation and transit of the tracer horseradish peroxidase. Quantification of interendothelial gaps revealed an eleven-fold increase with the Tx mimic relative to untreated endothial cells (p < 0.05) and prevention by pretreatment with the Tx receptor antagonist (p < 0.05). These data indicate that Tx directly modulates the permeability of endothelial cell in vitro.en_US
dc.description.sponsorshipNational Institutes of Health (GM24891-10, GM35141o03, HL16714-13); The U.S. Navy Office of Naval Research (N00014-79-C-0168); The Brigham Surgical Group, Inc.; The Trauma Research Foundation.en_US
dc.language.isoen
dc.publisherHindawi Publishing Corporationen_US
dc.rightsCopyright 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.titleThromboxane Modulates Endothelial Permeabilityen_US
dc.typeArticleen_US
dc.identifier.doi10.1155/S0962935194000190
dc.identifier.pmid18472934
dc.identifier.pmcid2367025


This item appears in the following Collection(s)

Show simple item record

Copyright 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as Copyright 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.