Show simple item record

dc.contributor.authorAlbergotti, Lori C.en_US
dc.contributor.authorHamlin, Heather J.en_US
dc.contributor.authorMcCoy, Michael W.en_US
dc.contributor.authorGuillette,, Louis J.en_US
dc.date.accessioned2012-01-11T16:55:00Z
dc.date.available2012-01-11T16:55:00Z
dc.date.issued2009-5-8
dc.identifier.citationAlbergotti, Lori C., Heather J. Hamlin, Michael W. McCoy, Louis J. Guillette. "Endocrine Activity of Extraembryonic Membranes Extends beyond Placental Amniotes" PLoS ONE 4(5): e5452. (2009)
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/2144/3134
dc.description.abstractBACKGROUND. During development, all amniotes (mammals, reptiles, and birds) form extraembryonic membranes, which regulate gas and water exchange, remove metabolic wastes, provide shock absorption, and transfer maternally derived nutrients. In viviparous (live-bearing) amniotes, both extraembryonic membranes and maternal uterine tissues contribute to the placenta, an endocrine organ that synthesizes, transports, and metabolizes hormones essential for development. Historically, endocrine properties of the placenta have been viewed as an innovation of placental amniotes. However, an endocrine role of extraembryonic membranes has not been investigated in oviparous (egg-laying) amniotes despite similarities in their basic structure, function, and shared evolutionary ancestry. In this study, we ask whether the oviparous chorioallantoic membrane (CAM) of chicken (Gallus gallus) has the capability to synthesize and receive signaling of progesterone, a major placental steroid hormone. METHODOLOGY/PRINCIPAL FINDINGS. We quantified mRNA expression of key steroidogenic enzymes involved in progesterone synthesis and found that 3β-hydroxysteroid dehydrogenase, which converts pregnenolone to progesterone exhibited a 464 fold increase in the CAM from day 8 to day 18 of embryonic development (F5, 68=89.282, p<0.0001). To further investigate progesterone synthesis, we performed explant culture and found that the CAM synthesizes progesterone in vitro in the presence of a steroid precursor. Finally, we quantified mRNA expression and performed protein immunolocalization of the progesterone receptor in the CAM. CONCLUSIONS/SIGNIFICANCE. Collectively, our data indicate that the chick CAM is steroidogenic and has the capability to both synthesize progesterone and receive progesterone signaling. These findings represent a paradigm shift in evolutionary reproductive biology by suggesting that endocrine activity of extraembryonic membranes is not a novel characteristic of placental amniotes. Rather, we hypothesize that these membranes may share an additional unifying characteristic, steroidogenesis, across amniotes at large.en_US
dc.description.sponsorshipSigma Xi (G20073141634396861); National Science Foundation (2008059161); UF-Howard Hughes G.A.T.O.R. Program; Howard Hughes Medical Institute Professorshipen_US
dc.language.isoen
dc.publisherPublic Library of Scienceen_US
dc.titleEndocrine Activity of Extraembryonic Membranes Extends beyond Placental Amniotesen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0005452
dc.identifier.pmid19424488
dc.identifier.pmcid2674930


This item appears in the following Collection(s)

Show simple item record