Prevalence of tetracycline resistance in veillonella; initial studies on developing a genetic system for veilonella
Al-Motlaq, Ibrahim M.
Henry M Goldman School of Dental Medicine (Boston University)
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Veillonella spp. are Gram-negative cocci that have been found to be amongst the most numerous anaerobes in human saliva. Veillonella spp. have an unusual physiology and special energy-conservation mechanisms that facilitate their growth in dental plaque. Unfortunately, the lack of a system for genetic manipulation of Veillonellae has severely hindered studies of this interesting group of organisms. In order to begin development of useful vectors, studies were carried out to identify antibiotic resistance genes from Veillonella spp. A pBluescript SK (-) phagemid lambda ZAP II library from Veillonella atypica PK1910 (TetR) was screened by plating on LB agar containing tetracycline. A phagemid conferring tetracycline resistance was isolated .. The phagemid contained a 6.6 kb DNA insert.The entire sequence of the insert was determined and it contained nine ORFs. All of the open reading frames were homologous to similar ORFs from the conjugative transposon Tn916 from Enterococcus faecalis including the tetracycline resistance gene, tetM. [TRUNCATED]
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact firstname.lastname@example.org.Thesis (D.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 2003 (Pediatric Dentistry).Includes bibliographical references (leaves 96-113).
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