Screening for serum inducible gene in sgordonii using TN 917-LAC mutagenesis
Al Silmi, Majeda
Henry M Goldman School of Dental Medicine (Boston University)
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S. gordonii, a member of the viridans streptococci group, is pioneer oral flora that initiates plaque formation on the tooth surface and a significant opportunistic pathogen causing bacteremia in susceptible patients. The transition from the tooth surface to the bloodstream presumably leads to alterations in gene expression. Previously, a library of S.gordonii Challis::Tn917-lacZ transcription mutants were constructed and tested for biofilm formation to identify biofilm defective mutants. In the current study, this library was used to develop a protocol to identify serum inducible gene/s in S. gordonii, to determine the influence of environmental factors on the expression of gene/s isolated and to identify serum components that induce altered gene expression. [TRUNCATED]
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact firstname.lastname@example.org.Thesis (M.S.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 2003 (Pediatric Dentistry).Includes bibliographic references (leaves 47-56).
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