Antimicrobial activity of Manuka honey as an intracanal medicament against E faecalis -- an in vitro study
Almarzooqi, Sameeha Husain Mohamed Husain
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The antibacterial activity against Enterococcus faecal is (E. faecalis) of 3 types of Manuka honey was investigated using indirect impedance microbiology, and their effectiveness was compared with that of the intracanal medicament calcium hydroxide [Ca(OH)2]. Methods: E. faecalis (ATCC 29212) inoculum was prepared and diluted to obtain 1 McF standard using spectrophotometer. Treatment groups were tested in triplicates and divided as the following: Manuka honeys MH 1 (n=3), MH2 (n=3), MH3 (n=3), Ca(OH) 2 (n=3), Positive control (n=3), Negative control (n=3). Test tubes with testing agents were placed in each RABIT insert cell as follows. 2.5 ml of TSB growth media were placed in each test tube. 2 g of the testing agent were placed in each corresponding tube except controls and vortex for few seconds. 0.1 g of dentin powder was added in each tube. Aliquots (0.5 ml) of the prepared E. faecalis broth dilution were added to the corresponding tube except the negative control. Prepared cells were placed in the incubator module.[TRUNCATED]
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact email@example.com.Thesis (MSD) --Boston University Institute for Dental Research and Education, Dubai, 2012 (Endodontics).Includes bibliography: leaves 86-105.
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